HrpG and HrpX Play Global Roles in Coordinating Different Virulence Traits of Xanthomonas axonopodis pv. citri
HrpG and HrpX Play Global Roles in Coordinating Different Virulence Traits of Xanthomonas axonopodis pv. citri
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DOI：
10.1094/mpmi-09-10-0209
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发表时间：
2011-06-01
影响因子：
3.5
通讯作者：
Wang, Nian
中科院分区：
文献类型：
--
作者：
Guo, Yinping;Figueiredo, Francisco;Wang, Nian
轴毛黄单胞菌 pv. citri 是柑橘溃疡病的病原体，柑橘溃疡病是柑橘最严重的病害之一。了解 X. axonopodis pv. 的毒力机制。 citri 中，我们设计并进行了全基因组微阵列分析，以表征 HrpG 和 HrpX 调节子，这对于 X. axonopodis pv. 的致病性至关重要。柑橘。我们的分析表明，232 个和 181 个基因分别属于 HrpG 和 HrpX 调节子。总共有 123 个基因在代表 X. axonopodis pv. 的三个生长阶段的三个选定时间点中的任何一个的两个调节子中重叠。 XVM2 培养基中的 citri。我们的结果表明，HrpG 和 HrpX 调节所有 24 个 III 型分泌系统基因、23 个 III 型分泌系统效应基因和 29 个 II 型分泌系统底物基因。我们的数据显示 X. axonopodis pv。 citri 调节响应宿主环境的多种细胞活动，例如氨基酸生物合成；氧化磷酸化；戊糖磷酸途径；糖、铁和钾的运输；通过 HrpX 和 HrpG 进行酚类分解代谢。我们发现 HrpG 和 HrpX 分别控制 124 个和 90 个未知基因。我们的结果表明，HrpG 和 HrpX 与全局信号网络相互作用，并协调多种毒力因子的表达，以改变和适应 X. axonopodis pv 期间的宿主环境。柑橘感染。
Xanthomonas axonopodis pv. citri is the causal agent of citrus canker, which is one of the most serious diseases of citrus. To understand the virulence mechanisms of X. axonopodis pv. citri, we designed and conducted genome-wide microarray analyses to characterize the HrpG and HrpX regulons, which are critical for the pathogenicity of X. axonopodis pv. citri. Our analyses revealed that 232 and 181 genes belonged to the HrpG and HrpX regulons, respectively. In total, 123 genes were overlapped in the two regulons at any of the three selected timepoints representing three growth stages of X. axonopodis pv. citri in XVM2 medium. Our results showed that HrpG and HrpX regulated all 24 type III secretion system genes, 23 type III secretion system effector genes, and 29 type II secretion system substrate genes. Our data revealed that X. axonopodis pv. citri regulates multiple cellular activities responding to the host environment, such as amino acid biosynthesis; oxidative phosphorylation; pentose-phosphate pathway; transport of sugar, iron, and potassium; and phenolic catabolism, through HrpX and HrpG. We found that 124 and 90 unknown genes were controlled by HrpG and HrpX, respectively. Our results suggest that HrpG and HrpX interplay with a global signaling network and co-ordinate the expression of multiple virulence factors for modification and adaption of host environment during X. axonopodis pv. citri infection.