Production and Characterization of a Monoclonal Antibody Against Spike Protein of Transmissible Gastroenteritis Virus
Production and Characterization of a Monoclonal Antibody Against Spike Protein of Transmissible Gastroenteritis Virus
复制标题
传染性胃肠炎病毒刺突蛋白单克隆抗体的制备和表征
DOI：
10.1089/hyb.2010.0009
复制
发表时间：
2010-08-01
期刊：
影响因子：
--
通讯作者：
Ren, Xiaofeng
中科院分区：
文献类型：
--
作者：
Meng, Fandan;Yin, Jiechao;Ren, Xiaofeng
传染性胃肠炎病毒（TGEV）是冠状病毒的一员。病毒刺突 (S) 蛋白介导 TGEV 与其易感细胞之间的相互作用。在这里，我们在原核系统中表达了编码 TGEV S 基因（称为 S1 基因）N 末端一半的截短基因。所得的 S1 蛋白用于免疫 BALB/c 小鼠，然后生成单克隆抗体 (MAb)。通过ELISA鉴定产生的MAb(7F9)并分析杂交瘤细胞的染色体数目。通过蛋白质印迹分析证实了 MAb 对 TGEV S 蛋白的免疫反应性。此外，免疫荧光测定表明 MAb 能够检测 TGEV 的细胞感染。本研究获得的MAb可作为检测TGEV S蛋白的特异性诊断试剂。
Transmissible gastroenteritis virus (TGEV) is a member of coronaviruses. The viral spike (S) protein mediates the interaction between TGEV and its susceptible cells. Here, we expressed a truncated gene encoding the N terminal half of TGEV S gene (designated S1 gene) in a prokaryotic system. The resulting S1 protein was used to immunize BALB/c mice followed by the generation of a monoclonal antibody (MAb). A generated MAb (7F9) was identified by ELISA and the chromosome number of the hybridoma cell was analyzed. The immunoreactivity of the MAb to TGEV S protein was confirmed by Western blot analysis. Moreover, immunofluorescence assays showed that the MAb is able to detect cell infection by TGEV. The MAb achieved in this study can be used as a specific diagnostic reagent for detecting TGEV S protein.