Leptospira mayottensis sp nov., a pathogenic species of the genus Leptospira isolated from humans
Leptospira mayottensis sp nov., a pathogenic species of the genus Leptospira isolated from humans
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DOI：
10.1099/ijs.0.066597-0
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发表时间：
2014-12-01
影响因子：
2.8
通讯作者：
Picardeau, Mathieu
中科院分区：
文献类型：
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作者：
Bourhy, Pascale;Collet, Louis;Picardeau, Mathieu
从马约特（印度洋）钩端螺旋体病患者中分离的代表钩端螺旋体属种的一组菌株先前被发现与其他已知的钩端螺旋体属种相当不同。这是从rrs（16 S rRNA）和其他遗传位点的序列分析中推断出来的，表明它们属于一个新物种。研究了目前在该新种属内鉴定的每个血清群的两种菌株。螺旋体，需氧，能动，螺旋形菌株生长良好，在30-37摄氏度，但不是在13摄氏度或在8-氮鸟嘌呤的存在下。还分析了菌株的基因组草案，以研究与钩端螺旋体属其他物种的DNA相关性。新的分离物形成了一个独特的分支，这是最密切相关的钩端螺旋体borgpetersenii，在多位点序列分析使用串联序列的基因rpoB，recA，fusA，gyrB，leuS和sucA。平均核苷酸的身份和基因组-基因组的距离，最近被提出作为可靠的替代经典的DNA-DNA杂交的分析，进一步证实，这些菌株应被归类为一个新的物种的代表。基因组DNA的G+C含量为39.5mol%。认为这些分离株代表一个新种，建议将其命名为马约腾钩端螺旋体新种，模式菌株为200901116（T）（=CIP 110703（T）=DSM 28999（T））。
A group of strains representing species of the genus Leptospira, isolated from patients with leptospirosis in Mayotte (Indian Ocean), were previously found to be considerably divergent from other known species of the genus Leptospira. This was inferred from sequence analysis of rrs (16S rRNA) and other genetic loci and suggests that they belong to a novel species. Two strains from each serogroup currently identified within this novel species were studied. Spirochaete, aerobic, motile, helix-shaped strains grew well at 30-37 degrees C, but not at 13 degrees C or in the presence of 8-azaguanine. Draft genomes of the strains were also analysed to study the DNA relatedness with other species of the genus Leptospira. The new isolates formed a distinct clade, which was most closely related to Leptospira borgpetersenii, in multilocus sequence analysis using concatenated sequences of the genes rpoB, recA, fusA, gyrB, leuS and sucA. Analysis of average nucleotide identity and genome-to-genome distances, which have recently been proposed as reliable substitutes for classical DNA DNA hybridization, further confirmed that these isolates should be classified as representatives of a novel species. The G+C content of the genomic DNA was 39.5 mol%. These isolates are considered to represent a novel species, for which the name Leptospira mayottensis sp. nov. is proposed, with 200901116(T) (=CIP 110703(T)=DSM 28999(T)) as the type strain.