Heme oxygenase-1 induced in Muller cells plays a protective role in retinal ischemia-reperfusion injury in rats
Heme oxygenase-1 induced in Muller cells plays a protective role in retinal ischemia-reperfusion injury in rats
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DOI：
10.1167/iovs.04-0450
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Yoshimura, N
中科院分区：
文献类型：
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作者：
Arai-Gaun, S;Katai, N;Yoshimura, N
目的。探讨血红素加氧酶(HO)-1和-2对大鼠视网膜缺血再灌注损伤的保护作用。通过将眼内压增加至 110 mm Hg 60 分钟来诱导大鼠视网膜缺血。通过蛋白质印迹、实时聚合酶链反应（PCR）和免疫组织化学测定视网膜中HO-1和-2的表达。为了抑制HO-1的上调，在缺血前玻璃体内注射HO-1的短干扰(si)RNA，并使用绿色荧光蛋白(GFP)作为对照。通过计数 S-100 阳性细胞的数量来评估 Muller 细胞损伤。通过计数ED-1阳性细胞的数量来确定侵入视网膜的巨噬细胞的数量。结果。 HO-1 mRNA和蛋白的表达在再灌注后6小时上调，并在12至24小时达到峰值，而HO-2的表达没有改变。再灌注24小时后，在Muller细胞中检测到HO-1免疫反应性，在视网膜细胞中检测到HO-2免疫反应性。与注射GFP的siRNA相比，再灌注后12小时和24小时，用HO-1的siRNA处理的视网膜中HO-I的表达降低。再灌注24小时后，用HO-1 siRNA处理的视网膜中S-100阳性细胞的数量显着减少（P
Purpose. To investigate the protective roles played by heme oxygenase (HO)-1 and -2 in the rat retina after ischemia-reperfusion injury.Methods. Retinal ischemia was induced in rats by increasing the intraocular pressure to 110 mm Hg for 60 minutes. The expression of HO-1 and -2 in the retina was determined by Western blot, real-time polymerase chain reaction (PCR), and immunohistochemistry. To inhibit the upregulation of HO-1, short interfering (si)RNA of HO-1 was injected intravitreally, before ischemia and that of green fluorescent protein (GFP) was used as the control. Muller cell damage was assessed by counting the number of S-100-positive cells. The number of macrophages invading the retina was determined by counting the number of ED-1-positive cells.Results. The expression of HO-1 mRNA and protein was upregulated at 6 hours after reperfusion and peaked at 12 to 24 hours, whereas that of HO-2 was not altered. HO-1 immunoreactivities were detected in Muller cells at 24 hours after reperfusion, and HO-2 immunoreactivities were detected in retinal cells. The HO-I expression in the retina treated with siRNA of HO-1 was reduced at 12 and 24 hours after reperfusion compared with that injected with siRNA of GFP. The number of S-100-positive cells at 24 hours after reperfusion decreased significantly in retinas treated with HO-1 siRNA (P