CUREing cancer: Development and implementation of a molecular biology‐focused course‐based undergraduate research experience using a cancer cell culture model
CUREing cancer: Development and implementation of a molecular biology‐focused course‐based undergraduate research experience using a cancer cell culture model
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治愈癌症：使用癌细胞培养模型开发和实施以分子生物学为基础的课程本科研究经验
DOI：
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发表时间：
2020
影响因子：
1.4
通讯作者：
Irene K Guttilla Reed
中科院分区：
文献类型：
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作者：
Irene K Guttilla Reed
许多理科学生有兴趣探索研究机会；然而，一对一的教师指导模式往往缺乏监督大量学生的能力。让本科生接触研究过程的另一种机制是参与基于课程的本科生研究体验（CURE）。 CURE 促进研究的包容性，为学生和教师提供结构，同时让学生参与科学发现。这项研究描述了癌症生物学中的 CURE 模型，并报告了学生的成果。学生们利用生物信息学来预测 miR-100 的靶基因，miR-100 是一种在乳腺癌转移的细胞培养模型中差异表达的 microRNA。学生们被要求查阅原始文献，为他们的目标基因撰写资助提案，然后接受培训以执行基本的分子生物学技术来测试他们的个人假设。此外，该课程还整合了解决实验问题和向小组展示数据的机会，并最终形成了一份出版风格的科学报告，讨论了他们个人研究项目的结果。学生们表示，根据评估前和评估后的调查，他们对执行各种分子生物学技术和研究相关技能的信心显着增强。学生反馈还表明，他们通过课程获得了对基础文献、实验设计和科学写作的理解。这项研究表明，CURE 可以成为一种有效的教学法，不仅可以让更多的学生参与研究，还可以提高他们在实验室的信心和技能。
Many students in the sciences are interested in exploring research opportunities; however, the one‐on‐one faculty mentorship model often lacks the ability to supervise large numbers of students. An alternative mechanism for exposing undergraduate students to the research process is participation in a Course‐based Undergraduate Research Experience (CURE). CUREs promote inclusivity in research, and provide structure for both students and faculty while engaging students in scientific discovery. This study describes a model for a CURE in cancer biology, and reports student outcomes. Students utilized bioinformatics to predict targets genes of miR‐100, a microRNA that is differentially expressed in a cell culture model of breast cancer metastasis. Students were required to engage with primary literature to write a grant proposal for their target gene, and then were trained to perform basic molecular biology techniques to test their individual hypotheses. Additionally, the course integrated opportunities to troubleshoot experiments and present data to the group, and culminated in a publication style scientific report discussing the results of their individual research project. Students reported significantly increased confidence in executing various molecular biology techniques and research‐related skills based on pre‐ and post‐assessment surveys. Student feedback also indicated that they gained an understanding of primary literature, experimental design, and scientific writing as a result of the course. This study supports that CUREs can be an effective pedagogy for not only engaging larger groups of students in research, but also improving their confidence and skill set in the laboratory.
影响因子：
11.2
作者：
Gaur, Arti;Jewell, David A.;Israel, Mark A.
通讯作者：
Israel, Mark A.