Role of sialyl 6-sulfo Lewis X in antitumor immunity against oral squamous cell carcinoma.
Role of sialyl 6-sulfo Lewis X in antitumor immunity against oral squamous cell carcinoma.
复制标题
唾液酸 6-磺基 Lewis X 在口腔鳞状细胞癌抗肿瘤免疫中的作用。
DOI：
10.1111/jop.12585
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发表时间：
2017
影响因子：
3.3
通讯作者：
Kobayashi M
中科院分区：
文献类型：
--
作者：
Yoshida H;Hoshino H;Imumura Y;Yoshimura H;Sano K;Kobayashi M
肿瘤浸润淋巴细胞（TIL）在口腔鳞状细胞癌（OSCC）的抗肿瘤免疫中起着重要作用，但其募集机制尚不清楚。本研究旨在评估TIL和表达唾液酸6-磺基刘易斯X（LeX）的高内皮小静脉（HEV）样血管之间的潜在关联。方法对口腔鳞癌组织切片（n=41）进行唾液酸6-磺基LeX和CD 34的免疫组化，以定量HEV样血管。进行唾液酸6-磺基LeX和CD 3和CD 20或CD 4和CD 8的三重免疫组化，以确定哪种淋巴细胞亚群与HEV样血管更密切相关。（65.9%），且在早期（T1/T2期）较晚期（T3/T4期）多见。附着在这些HEV样血管内表面的T细胞数量显著大于B细胞，而CD 4+辅助T细胞和CD 8+细胞毒性T细胞的数量没有显著差异。有趣的是，唾液酸6-磺基LeX也表达在一部分OSCC细胞的膜上，并且几乎只发现CD 8+细胞毒性T细胞附着于这些癌cells.ConclusionsSialyl 6-磺基LeX不仅在HEV样血管上显示，而且在OSCC细胞上显示，并且可能在针对OSCC的抗肿瘤免疫中发挥潜在作用。
BackgroundTumor‐infiltrating lymphocytes (TILs) reportedly play a pivotal role in antitumor immunity against oral squamous cell carcinoma (OSCC); however, mechanisms governing TIL recruitment to OSCC tissues remain to be clarified. This study was undertaken to assess a potential association between TILs and high endothelial venule (HEV)‐like vessels that express sialyl 6‐sulfo Lewis X (LeX).MethodsOSCC tissue sections (n=41) were subjected to immunohistochemistry for sialyl 6‐sulfo LeX and CD34 to allow quantitation of HEV‐like vessels. Triple immunohistochemistry for sialyl 6‐sulfo LeX and either CD3 and CD20 or CD4 and CD8 was conducted to determine which lymphocyte subset is more closely associated with HEV‐like vessels.ResultsHEV‐like vessels expressing sialyl 6‐sulfo LeX were detected in 27 of 41 (65.9%) OSCC cases, and these vessels were more frequently found in early disease (T1/T2 stages) compared with advanced (T3/T4) stages. The number of T cells attached to the inner surface of these HEV‐like vessels was significantly greater than that of B cells, while the number of CD4+helper T cells and CD8+cytotoxic T cells did not differ significantly. Interestingly, sialyl 6‐sulfo LeX was also expressed on the membrane of a fraction of OSCC cells, and CD8+cytotoxic T cells were almost exclusively found attached to these carcinoma cells.ConclusionsSialyl 6‐sulfo LeX is displayed not only on HEV‐like vessels but also on OSCC cells and may potentially function in antitumor immunity against OSCC.