INTEGRIN ADHESION MOLECULES IN THE HUMAN ENDOMETRIUM - CORRELATION WITH THE NORMAL AND ABNORMAL MENSTRUAL-CYCLE
INTEGRIN ADHESION MOLECULES IN THE HUMAN ENDOMETRIUM - CORRELATION WITH THE NORMAL AND ABNORMAL MENSTRUAL-CYCLE
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DOI：
10.1172/jci115835
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发表时间：
1992-07-01
影响因子：
15.9
通讯作者：
BUCK, CA
中科院分区：
文献类型：
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作者：
LESSEY, BA;DAMJANOVICH, L;BUCK, CA
整合素是一类参与细胞-细胞和细胞-基质相互作用的细胞黏附分子，几乎存在于所有人类细胞中。用免疫过氧化物酶染色方法检测9种不同的α、β整合素亚基在月经周期不同阶段人子宫内膜组织中的分布。腺上皮细胞主要表达α-2、α-3和α-6(胶原/层粘连蛋白受体)，而基质细胞主要表达α-5(纤维连接蛋白受体)。腺上皮细胞上α-1的存在是周期特异性的，仅在分泌期才能发现。玻璃连结蛋白受体的两个亚单位α(V)β-3在子宫内膜上皮细胞中的表达也经历了周期特异性的变化。在整个月经周期中，α(V)的免疫染色增强，而β-3亚单位在周期第20天突然出现在腔上皮细胞和腺上皮细胞上。不孕症患者的黄体期活检(大于或等于3d的“异相”)显示延迟的上皮β-3免疫染色。这些结果表明，子宫内膜与其他上皮组织既有相似之处，也有特殊的区别。某些整合素部分似乎在周期子宫内膜中受到调节，并破坏整合素的表达。可能与子宫容受性下降和不孕不育有关。
Integrins are a class of cell adhesion molecules that participate in cell-cell and cell-substratum interactions and are present on essentially all human cells. The distribution of nine different alpha and beta-integrin subunits in human endometrial tissue at different stages of the menstrual cycle was determined using immunoperoxidase staining. Glandular epithelial cells expressed primarily alpha-2, alpha-3, and alpha-6 (collagen/laminin receptors), while stromal cells expressed predominantly alpha-5 (fibronectin receptor). The presence of alpha-1, on glandular epithelial cells was cycle specific, found only during the secretory phase. Expression of both subunits of the vitronectin receptor, alpha(v)beta-3, also underwent cycle specific changes on endometrial epithelial cells. Immunostaining for alpha(v) increased throughout the menstrual cycle, while the beta-3 subunit appeared abruptly on cycle day 20 on luminal as well as glandular epithelial cells. Discordant luteal phase biopsies (greater-than-or-equal-to 3 d "out of phase") from infertility patients exhibited delayed epithelial beta-3 immunostaining. These results demonstrate similarities, as well as specific differences, between endometrium and other epithelial tissues. Certain integrin moieties appear to be regulated within the cycling endometrium and disruption of integrin expression. may be associated with decreased uterine receptivity and infertility.