Isolation, structural characterization, and lymphopoiesis stimulant activity of a polysaccharide from the abalone gonad
Isolation, structural characterization, and lymphopoiesis stimulant activity of a polysaccharide from the abalone gonad
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鲍鱼性腺多糖的分离、结构表征和淋巴细胞生成刺激活性
DOI：
10.1007/s10068-015-0004-4
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发表时间：
2015-02-01
影响因子：
2.9
通讯作者：
Zhu, Bei-wei
中科院分区：
文献类型：
--
作者：
Yang, Jing-feng;Li, Yue-hui;Zhu, Bei-wei
以皱纹盘鲍（Haliotis discus hannaiIno）性腺为原料，采用蛋白酶辅助分离、离子交换层析和凝胶过滤层析等方法，从其性腺中分离得到一种新的多糖（AGP-32）。AGP-32的骨架使用三氟乙酸水解来测定。FTIR、NMR和甲基化分析以及高碘酸氧化和Smith降解分析表明，AGP-32的主链主要由（1→6）-连接的甘露糖、（1→3）-连接的半乳糖和（1→3）-连接的葡萄糖组成，比例为2：3：1。体外细胞增殖实验表明，浓度为50 μg/mL的AGP-32对小鼠脾淋巴细胞增殖有26%的促进作用。AGP-32具有免疫保护作用，是考虑作为功能性食品的候选者。
A novel polysaccharide (AGP-32) from the gonad ofHaliotis discus hannaiIno was isolated using a protease-assisted process and successive ion-exchange and gel-filtration chromatography. The backbone of AGP-32 was determined using hydrolysis with trifluoroacetic acid. FTIR, NMR, and methylation analysis, and periodate oxidation and Smith degradation analysis revealed that the AGP-32 backbone mainly consisted of (1→6)-linked mannose, (1→3)-linked galactose, and (1→3)-linked glucose in a proportion of 2:3:1. Anin vitrocell assay indicated that AGP-32 promoted mice splenic lymphocyte proliferation by 26% at a concentration of 50 μg/mL. AGP-32 had an effect on immune protection and is a candidate for consideration as a functional food.