Dual RNA-Sequencing and Liquid Chromatography-Mass Spectrometry Unveil Specific Insights on the Pathogenicity of Trichophyton mentagrophytes Complex.
Dual RNA-Sequencing and Liquid Chromatography-Mass Spectrometry Unveil Specific Insights on the Pathogenicity of Trichophyton mentagrophytes Complex.
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DOI：
10.1016/j.jid.2022.08.055
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
Yubo Ma;Weiwei Deng;Kai Zhang;Yinggai Song;Lu Zhang;Jin Shao;Xiao Liu;Z. Wan;Xiaowen Wang;Ruoyu Li
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文献类型：
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作者：
Yubo Ma;Weiwei Deng;Kai Zhang;Yinggai Song;Lu Zhang;Jin Shao;Xiao Liu;Z. Wan;Xiaowen Wang;Ruoyu Li
须发癣越来越被认为是一个公共卫生的危害，因为它会导致最严重的表现皮肤真菌病。在本研究中，我们进行了一系列的研究，以确定的致病性的T. mentagrophytescomplex。我们发现须癣毛癣菌复合物通过模式识别受体MAPK/非经典NF-κB途径与角质形成细胞相互作用，并且须癣毛癣菌的菌丝形式负责角质形成细胞中增加的炎症反应。此外，SN-38可能是须癣毛癣菌的一种毒素，在体内和体外均可诱导角质形成细胞凋亡。我们的研究结果解释了临床上观察到的须癣毛癣菌的严重致病性和破坏性，并为设计新型毒素导向疗法以改善患者预后铺平了道路。
Trichophytonmentagrophytesis increasingly considered to be a public health hazard because it causes the most severe manifestations of dermatophytosis. In this study, we performed a series of studies to determine the pathogenicity of theT. mentagrophytescomplex. We show that theT. mentagrophytescomplex interacts with keratinocytes through pattern-recognition receptors‒MAPK/noncanonical NF-κB pathways and that the hyphal form ofT. mentagrophytesis responsible for the increased inflammatory responses in keratinocytes. Moreover, SN-38 is likely a toxin ofT. mentagrophytesthat induces apoptosis in keratinocytes both in vivo and in vitro. Our results explain the severe pathogenicity and destructiveness ofT. mentagrophytesobserved in the clinic and pave the way for designing novel toxin-directed therapies to improve patient outcomes.