AcstuA, which encodes an APSES transcription regulator, is involved in conidiation, cephalosporin biosynthesis and cell wall integrity of Acremonium chrysogenum
AcstuA, which encodes an APSES transcription regulator, is involved in conidiation, cephalosporin biosynthesis and cell wall integrity of Acremonium chrysogenum
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AcstuA 编码 APSES 转录调节因子，参与产黄顶孢霉的分生孢子形成、头孢菌素生物合成和细胞壁完整性。
DOI：
10.1016/j.fgb.2015.08.003
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发表时间：
2015-10-01
影响因子：
3
通讯作者：
Liu, Gang
中科院分区：
文献类型：
--
作者：
Hu, Pengjie;Wang, Ying;Liu, Gang
从产黄枝顶孢霉（Acremonium chrysogenum）中鉴定出一个转录调控基因AcstuA。AcstuA编码一种与StuA相似的碱性螺旋-环-螺旋蛋白，调节构巢曲霉的核心发育过程。就像A中stuA的中断。AcstuA的缺乏通过严重下调AcbrlA和AcabaA的表达来阻断产黄曲霉的产孢，AcbrlA和AcabaA编码关键真菌发育调节因子BrlA和AbaA的直系同源物。AcstuA的破坏也大大降低了A.产金菌AcstuA破坏突变体（Delta AcstuA）中pcbAB、pbcC、cefD 1、cefD 2、cefEF和cefG的转录水平显著降低。除了分生孢子和头孢菌素生物合成的缺陷，三角洲AcstuA产生异常肿胀和破碎的菌丝细胞在发酵过程中。AcstuA缺失引起的菌丝细胞表型改变可通过添加NaCl得到恢复，表明AcstuA缺失影响了菌丝细胞壁的完整性。产金菌两个推定的甘露糖蛋白编码基因Acmp 2和Acmp 3的转录在Delta AcstuA中显著降低，进一步表明突变体的细胞壁完整性受损。这些结果表明AcstuA基因与A.产金菌(C)2015 Elsevier Inc. All rights reserved.
A transcriptional regulatory gene AcstuA was identified from Acremonium chrysogenum. AcstuA encodes a basic helix-loop-helix protein with similarity to StuA which regulates the core developmental processes of Aspergillus nidulans. Like disruption of stuA in A. nidulans, deficiency of AcstuA blocked the conidiation of A chrysogenum through severely down-regulating the expression of AcbrlA and AcabaA which encode orthologs of the key fungal developmental regulators BrlA and AbaA. Disruption of AcstuA also drastically reduced cephalosporin production of A. chrysogenum. In agreement, the transcriptions of pcbAB, pbcC, cefD1, cefD2, cefEF and cefG were remarkably decreased in the AcstuA disruption mutant (Delta AcstuA). In addition to defects in conidiation and cephalosporin biosynthesis, Delta AcstuA produced abnormal swollen and fragmented hyphal cells during fermentation. The phenotypic alterations of hyphal cells caused by AcstuA deletion were restored by supplementation of NaCl in the medium, indicating that the deficiency of AcstuA has an influence on the cell wall integrity of A. chrysogenum. The transcriptions of two putative mannoprotein encoding genes Acmp2 and Acmp3 significantly reduced in Delta AcstuA, further indicating that cell wall integrity of the mutant is impaired. These results strongly suggested that AcstuA is involved in conidiation, cephalosporin production, hyphal fragmentation and cell wall integrity in A. chrysogenum. (C) 2015 Elsevier Inc. All rights reserved.