Unravelling complex transposable elements surrounding blaGES-16 in a Pseudomonas aeruginosa ExoU strain.
Unravelling complex transposable elements surrounding blaGES-16 in a Pseudomonas aeruginosa ExoU strain.
复制标题
解开铜绿假单胞菌 ExoU 菌株中 blaGES-16 周围的复杂转座元件。
DOI：
10.1016/j.jgar.2022.04.009
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发表时间：
2022
影响因子：
4.6
通讯作者：
Gales,AnaC
中科院分区：
文献类型：
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作者：
Streling,AnaPaula;Cayô,Rodrigo;Catan,ThaisA;Jové,Thomas;Santos,FernandaF;Nodari,CarolinaS;Hanson,Blake;Miller,WilliamR;Shropshire,William;Dinh,AnQ;Ribeiro,Julival;Pignatari,AntonioCC;Arias,CesarA;Gales,AnaC
目的对巴西一株铜绿假单胞菌外显子(P-10.226)中blaGES-16的复杂周边区域进行特征分析。方法采用MALDITOFMS进行菌种鉴定，并根据欧洲药敏试验委员会(EUCAST)的折点，采用肉汤微量稀释法测定药敏图谱。对P-10.226菌株的全基因组测序结果表明，P-10.226携带BLES-16基因，该基因于1992年插入到一个新的I类整合子中(aadB-blaOXA-56-blages-16-aadB-aadA6c)，其3‘-CS被嵌套的转座元件IS5564：ISpa157截断。自从1992年在下游发现了IS6100-ΔIS6100结构和含有操纵子RTPADE的类似TnAs2的结构以来，结构更加复杂。1992年发现的含有25bp不完全反向重复序列的TnAs3片段位于IS6100-ΔIS6100的两侧，这可能是其先前存在于基因组中的遗传标志。有趣的是，1992年还显示了与IN581(blages-16-dfrA22-aacA27-aadA1)不同的盒式磁带阵列，这是以前在巴西发现的粘质沙雷氏菌菌株中报告的。最后，exoUgene，它编码了来自P的一种有效的III型分泌系统(T3SS)效应蛋白的细胞毒素。铜绿假单胞菌与严重感染有关，也被检测到。结论我们在1992年描述了这一新的携带XDRP中被复杂转位事件包围的blaGES-16。铜绿假单车。与TnAs3片段相邻的许多组直接重复序列的鉴定表明，在1992年形成当前遗传环境的转位事件是一个重要的过去。
ObjectivesWe characterised the complex surrounding regions ofblaGES-16in aPseudomonas aeruginosa exoU+ strain (P-10.226) in Brazil.MethodsSpecies identification was performed by MALDI-TOF MS, and the antimicrobial susceptibility profile was determined by broth microdilution based on European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints. The whole genome sequencing (WGS) of P-10.226 strain was performed using both short-read paired-end sequencing on the Illumina MiSeq platform as well as the long-read Oxford Nanopore MinION.ResultsWGS analysis showed that P-10.226 carriedblaGES-16, which was found as a gene cassette inserted into a novel class I integron, In1992 (aadB-blaOXA-56-blaGES-16-aadB-aadA6c), whose 3′-CS was truncated by a nested transposable element, IS5564::ISPa157. The structure was even more complex since IS6100-ΔIS6100structure and a TnAs2-like harbouring the operonmerRTPADEwas found downstream In1992. Fragments of TnAs3harbouring 25-bp imperfect inverted repeats were identified bordering theintl1of In1992 and also flanking IS6100-ΔIS6100, which might be genetic marks of its previous presence in the genome. Interestingly, In1992 also shows a distinct cassette array from In581 (blaGES-16-dfrA22-aacA27-aadA1), which was previously reported inSerratia marcescensstrains recovered in Brazil. Finally,exoUgene, which encodes a potent cytotoxin of type III secretion systems (T3SS) effector proteins fromP. aeruginosaand is associated to severe infections, was also detected.ConclusionWe described the novel In1992 carryingblaGES-16surrounded by complex transposition events in a XDRP. aeruginosastrain. The identification of many sets of direct repeats adjacent to TnAs3fragments indicates a major past of transposition events that shaped the current genetic environment of In1992.