Structural and functional analysis of SGT1-HSP90 core complex required for innate immunity in plants.
Structural and functional analysis of SGT1-HSP90 core complex required for innate immunity in plants.
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DOI：
10.1038/embor.2008.185
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发表时间：
2008-12
期刊：
影响因子：
7.7
通讯作者：
Shirasu, Ken
中科院分区：
文献类型：
--
作者：
Kadota, Yasuhiro;Amigues, Beatrice;Ducassou, Lionel;Madaoui, Hocine;Ochsenbein, Francoise;Guerois, Raphael;Shirasu, Ken
SGT1（skp1 的 G2 等位基因的抑制因子）是 HSP90（热休克蛋白 90）的辅助伴侣，是植物和动物先天免疫所必需的。揭示 SGT1 与其他共伴侣（如 p23、AHA1（HSP90 ATPase 1 激活剂）或 RAR1（Mla12 抗性所需））之间的串扰是了解 HSP90 机制的重要一步。 HSP90 的核磁共振波谱和突变分析揭示了其与 SGT1 的 CS 结构域结合的性质。尽管 CS 在结构上与 p23 相似，但这些结构域被发现与 HSP90 的各个区域非竞争性结合；然而，出乎意料的是，全长 SGT1 可以取代 HSP90 中的 p23。 RAR1 与 HSP90 部分共享相同的 CS 结构域结合位点，而 AHA1 则不然。该分析使我们能够构建 HSP90-SGT1 复合物的结构模型，并获得双方之间的补偿突变对，该突变对能够通过 Rx（马铃薯病毒 X 抗性）免疫传感器稳定来恢复体内病毒抗性。
SGT1 (Suppressor of G2 allele of skp1), a co-chaperone of HSP90 (Heat-shock protein 90), is required for innate immunity in plants and animals. Unveiling the cross talks between SGT1 and other co-chaperones such as p23, AHA1 (Activator of HSP90 ATPase 1) or RAR1 (Required for Mla12 resistance) is an important step towards understanding the HSP90 machinery. Nuclear magnetic resonance spectroscopy and mutational analyses of HSP90 revealed the nature of its binding with the CS domain of SGT1. Although CS is structurally similar to p23, these domains were found to non-competitively bind to various regions of HSP90; yet, unexpectedly, full-length SGT1 could displace p23 from HSP90. RAR1 partly shares the same binding site with HSP90 as the CS domain, whereas AHA1 does not. This analysis allowed us to build a structural model of the HSP90–SGT1 complex and to obtain a compensatory mutant pair between both partners that is able to restore virus resistance in vivo through Rx (Resistance to potato virus X) immune sensor stabilization.
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影响因子：
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作者：
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DOI：
10.1073/pnas.0610926104
发表时间：
2007-04-17
影响因子：
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作者：
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通讯作者：
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