Red/Near-Infrared Emissive Metalloporphyrin-Based Nanodots for Magnetic Resonance Imaging-Guided Photodynamic Therapy In Vivo
Red/Near-Infrared Emissive Metalloporphyrin-Based Nanodots for Magnetic Resonance Imaging-Guided Photodynamic Therapy In Vivo
复制标题
用于磁共振成像引导体内光动力治疗的红色/近红外发射金属卟啉纳米点
DOI：
10.1002/ppsc.201800208
复制
发表时间：
2018
影响因子：
2.7
通讯作者：
Zhu Xunjin
中科院分区：
文献类型：
--
作者：
Wu Fengshou;Chen Jingwen;Li Zhicong;Su Huifang;Leung Ken Cham-Fai;Wang Han;Zhu Xunjin
近红外发射（NIR）卟啉植入碳纳米点（PCND或MPCND）通过在柠檬酸存在下选择性碳化四（meso-aminophenyl）卟啉的游离碱或金属络合物[M = Zn（II）或Mn（III）]来制备。所制备的纳米点表现出自发的NIR发射、小尺寸、良好的水溶性以及卟啉和原始碳纳米点两者的有利生物相容性特征。纳米点的亚细胞定位实验表明了溶酶体靶向特征。对HeLa细胞的体外光动力学治疗（PDT）结果表明，单独的纳米点对肿瘤细胞没有不良影响，但在照射后显示出显着的光动力学效应。此外，含有顺磁性Mn（III）离子的MnPCNDs具有良好的生物相容性、NIR发光和磁共振成像以及高效的单线态氧产生，在体内磁共振成像引导的光动力学治疗中进行了进一步研究。
Near‐infrared emissive (NIR) porphyrin‐implanted carbon nanodots (PCNDs or MPCNDs) are prepared by selectively carbonization of free base or metal complexes [M = Zn(II) or Mn(III)] of tetra‐(meso‐aminophenyl)porphyrin in the presence of citric acid. The as‐prepared nanodots exhibit spontaneously NIR emission, small size, good aqueous dispersibility, and favorable biocompatibility characteristic of both porphyrins and pristine carbon nanodots. The subcellular localization experiment of nanodots indicates a lysosome‐targeting feature. And the in vitro photodynamic therapy (PDT) results on HeLa cells indicate the nanodots alone have no adverse effect on tumor cells, but display remarkable photodynamic efficacy upon irradiation. Moreover, MnPCNDs containing paramagnetic Mn(III) ions, which possesses good biocompatibility, NIR luminescence, and magnetic resonance imaging and efficient singlet oxygen production, are further studied in magnetic resonance imaging‐guided photodynamic therapy in vivo.