Author Response: Postnatal Overexpression of the Human ARMS2 Gene Does Not Induce Abnormalities in Retina and Choroid in Transgenic Mouse Models.
Author Response: Postnatal Overexpression of the Human ARMS2 Gene Does Not Induce Abnormalities in Retina and Choroid in Transgenic Mouse Models.
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作者回应：在转基因小鼠模型中，人类 ARMS2 基因的出生后过度表达不会引起视网膜和脉络膜异常。
DOI：
10.1167/iovs.14-16174
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Iwata T.
中科院分区：
文献类型：
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作者：
曽良一郎;佐々木一益;住吉晃;野中博意;笠原好之;池田和隆;渡辺雅彦;川島隆太;Iwata T.
三个独立的研究小组已经表明，HTRA1的过表达显著影响脉络膜毛细血管，从而导致息肉样脉络膜血管病变（PCV）或脉络膜新生血管（CNV）。此外，两个独立的研究小组现在已经表明，ARMS2的过表达对视网膜没有任何影响。正如Liu和Hoh1所评论的那样，我们的实验不是为了观察HTRA1和ARMS2作为蛋白质或RNA的相互作用。我们目前正在研究与ARMS2基因序列相关的HTRA1启动子和调控元件。我们还在开发一个敲入小鼠模型，使用几个不同的启动子敲入Rosa 26位点的单个人类HTRA1 cDNA。我们希望在不久的将来报道这一点。
Three independent groups have now shown that overexpression of HTRA1 significantly effects the choroidal capillary, which leads to polypoidal choroidal vasculopathy (PCV) or choroidal neovascularization (CNV). Additionally, two independent groups have now shown that overexpression of ARMS2 does not have any effect in the retina. As Liu and Hoh1 have commented, our experiment was not aimed to observe interaction of HTRA1 and ARMS2 as protein or RNA. We are now in progress to study HTRA1 promoter and regulatory elements in relation to ARMS2 gene sequence. We are also in development of a knockin mouse model of a single human HTRA1 cDNA in Rosa 26 locus using several different promoters. We hope to report this in the near future.