High osmolarity extends life span in Saccharomyces cerevisiae by a mechanism related to calorie restriction
High osmolarity extends life span in Saccharomyces cerevisiae by a mechanism related to calorie restriction
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DOI：
10.1128/mcb.22.22.8056-8066.2002
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发表时间：
2002-11-01
影响因子：
5.3
通讯作者：
Guarente, L
中科院分区：
文献类型：
--
作者：
Kaeberlein, M;Andalis, AA;Guarente, L
热量限制（CR）延长了许多不同生物的寿命，包括哺乳动物。我们在这里描述了一种新的途径，该途径延长了酿酒酵母母细胞的寿命，但不涉及培养基卡路里含量的降低，即，酵母细胞在高浓度的外部渗透剂存在下生长。像CR一样，这种对高渗透压的长寿促进反应需要SIR2，这表明了寿命调节的共同机制。遗传和微阵列分析表明，高渗透压通过激活Hog1p延长寿命，导致糖酵解中间产物甘油生物合成的增加。这种代谢转变可能会增加NAD水平，从而激活Sir2p并促进长寿。
Calorie restriction (CR) extends life span in many different organisms, including mammals. We describe here a novel pathway that extends the life span of Saccharomyces cerevisiae mother cells but does not involve a reduction in caloric content of the media, i.e., there is growth of yeast cells in the presence of a high concentration of external osmolytes. Like CR, this longevity-promoting response to high osmolarity requires SIR2, suggesting a common mechanism of life span regulation. Genetic and microarray analysis indicates that high osmolarity extends the life span by activating Hog1p, leading to an increase in the biosynthesis of glycerol from glycolytic intermediates. This metabolic shift likely increases NAD levels, thereby activating Sir2p and promoting longevity.