Endochondral calcification by hypertrophic chondrocytes in the Meckel's cartilage grafted into the isogenic mouse spleen.
Endochondral calcification by hypertrophic chondrocytes in the Meckel's cartilage grafted into the isogenic mouse spleen.
复制标题
移植到同基因小鼠脾脏中的梅克尔软骨中的肥大软骨细胞导致软骨内钙化。
DOI：
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发表时间：
1990
影响因子：
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通讯作者：
T. Nawa
中科院分区：
文献类型：
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作者：
K. Ishizeki;N. Fujiwara;M. Sugawara;T. Nawa
我们发现，将妊娠第18天取自小鼠的Meckel‘s软骨条中段移植到同基因小鼠的脾中，软骨细胞诱导软骨内钙化。在整个Meckel‘s软骨基质开始钙化的同时，在软骨细胞周围观察到周期性的带状粗大的胶原纤维和基质小泡。虽然移植前的软骨细胞大多是肥大的细胞，但它们在脾组织中存活了7天，并具有发达的分泌细胞器。被钙化基质包裹的细胞相对较小，呈球形，形态与骨细胞相似。这些发现表明，肥大的软骨细胞的寿命受到脾微环境的影响。
We found that when the midsection of Meckel's cartilage bars obtained from mice on the eighteenth day of gestation were grafted into isogenic mouse spleen, chondrocytes induced an endochondral calcification. Concurrent with the onset of calcification throughout Meckel's cartilage matrix, periodic banded thick collagen fibrils and matrix vesicles were observed around the chondrocytes. Although most of the chondrocytes prior to grafting were hypertrophic cells, they survived for seven days in the splenic tissue and had well-developed secretory organelles. The cells which were surrounded by calcified matrix were relatively small, spherical, and showed a morphology closely resembling that of osteocytes. These findings suggest that the life span of hypertrophic chondrocytes is influenced by the microenvironment of the spleen.