EPIDERMAL GROWTH-FACTOR (EGF) EXPRESSION IN THE CONGENITAL POLYCYSTIC MOUSE KIDNEY
EPIDERMAL GROWTH-FACTOR (EGF) EXPRESSION IN THE CONGENITAL POLYCYSTIC MOUSE KIDNEY
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DOI：
10.1038/ki.1991.7
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发表时间：
1991-01-01
影响因子：
19.6
通讯作者：
KLOTMAN, PE
中科院分区：
文献类型：
--
作者：
HORIKOSHI, S;KUBOTA, S;KLOTMAN, PE
表皮生长因子在先天性多囊小鼠肾脏中的表达尽管细胞代谢、基底膜成分和生长因子异常已被提出，但先天性多囊肾病的肾囊肿形成机制尚不清楚。在目前的研究中，我们研究了表皮生长因子(EGF)在小鼠模型囊性形成中的潜在作用。我们检测了来自先天性多囊肾病(CPK)小鼠的肾囊液、尿液和血清中生长因子的活性和浓度。在受影响的小鼠中，尿液中的生长因子活性和尿EGF浓度都比未受影响的小鼠低得多，即使在校正了肌酐浓度后也是如此。虽然感染小鼠的生长因子活性较低，但在囊性动物中EGF的区域分布存在显著差异。囊液中的生长因子活性和EGF浓度均高于尿液。以BALB/MK上皮角质形成细胞为靶细胞，抗EGF抗体可完全抑制囊内生长因子活性。与仔鼠对照组相比，感染小鼠肾脏中EGF mRNA的表达显著降低。这些结果表明，EGF产生减少和EGF浓度的局部差异可能是导致包囊形成的原因。
Epidermal growth factor (EGF) expression in the congenital polycystic mouse kidney. The mechanisms responsible for renal cyst formation in congenital polycystic kidney disease remain unknown, although abnormalities of cellular metabolism, basement membrane components, and growth factors have been suggested. In the present study, we examined a potential role for epidermal growth factor (EGF) in cyst formation in a mouse model. We measured growth factor activity and concentration in renal cyst fluid, urine, and serum obtained from mice with congenital polycystic kidney disease (cpk). In affected mice, both growth factor activity of urine and the urinary EGF concentration were much lower than unaffected littermate controls even when corrected for creatinine concentration. Although the growth factor activity was much lower in affected mice, there were significant differences in the regional distribution of EGF in animals with cysts. Both growth factor activity and EGF concentration were greater in cyst fluid when compared to urine. Growth factor activity in cysts was completely inhibited by anti-EGF antibody using BALB/MK epithelial keratinocytes as targets. The expression of EGF mRNA in kidneys from affected mice was markedly decreased when compared to littermate controls. Theses results suggest that decreased EGF production and local differences in EGF concentration may contribute to cyst formation.