GENETIC SELECTION FOR MUTATIONS THAT IMPAIR THE COOPERATIVE BINDING OF LAMBDA REPRESSOR
GENETIC SELECTION FOR MUTATIONS THAT IMPAIR THE COOPERATIVE BINDING OF LAMBDA REPRESSOR
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DOI：
10.1111/j.1365-2958.1994.tb00337.x
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发表时间：
1994-02-01
影响因子：
3.6
通讯作者：
YOUDERIAN, P
中科院分区：
文献类型：
--
作者：
BENSON, N;ADAMS, C;YOUDERIAN, P
噬菌体 lambda 阻遏蛋白与相邻的 DNA 靶位点对协同结合。一种新颖的正向遗传选择组合，涉及源自 P22 攻击噬菌体的两种不同操纵子融合，用于分离突变型 lambda 阻遏物，这些阻遏物失去了与串联位点协同结合的能力，但保留了结合强单位点的能力。这些 cb（协同结合）突变导致 10 种不同的氨基酸变化，从而定义了阻遏蛋白羧基末端的 8 个残基。因为攻击噬菌体衍生物可用于研究基本上任何特定的蛋白质-DNA相互作用，所以遗传选择的类似组合可用于探索多种蛋白质相互作用以组装调节复合物的方式。
Bacteriophage lambda repressor binds co-operatively to adjacent pairs of DNA target sites. A novel combination of positive genetic selections, involving two different operon fusions derived from P22 challenge phages, was used to isolate mutant lambda repressors that have lost the ability to bind co-operatively to tandem sites yet retain the ability to bind a strong, single site. These cb (co-operative binding) mutations result in 10 different amino acid changes, which define eight residues in the carboxyl-terminus of repressor. Because challenge phage derivatives may be applied to study essentially any specific protein-DNA interaction, analogous combinations of genetic selections may be used to explore the ways that a variety of proteins interact to assemble regulatory complexes.