Temporal Coordination of Collective Migration and Lumen Formation by Antagonism between Two Nuclear Receptors
Temporal Coordination of Collective Migration and Lumen Formation by Antagonism between Two Nuclear Receptors
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DOI：
10.1016/j.isci.2020.101335
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发表时间：
2020-03
期刊：
影响因子：
5.8
通讯作者：
Xianping Wang;Heng Wang;Lin Liu;Sheng Li;G. Emery;Jiong Chen
中科院分区：
文献类型：
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作者：
Xianping Wang;Heng Wang;Lin Liu;Sheng Li;G. Emery;Jiong Chen
在发育过程中，细胞经历多个不同的形态发生过程以形成组织或器官，但它们的时间顺序和时间间隔如何确定仍然知之甚少。在这里，我们表明核受体E75和DHR 3调节果蝇胚胎发生过程中一组称为边缘细胞的连贯细胞的集体迁移和腔形成之间的时间顺序和时间间隔。我们发现，E75，在响应蜕皮激素信号，拮抗DHR 3的活动在边缘细胞迁移，和DHR 3是必要的和足够的后续腔的形成是至关重要的珠孔形态发生。DHR 3的管腔诱导功能主要通过另一种核受体和转录因子βFtz-f1介导。此外，DHR 3和βFtz-f1都是几丁质分泌到管腔中所需的，而DHR 3足以分泌几丁质。最后，DHR 3和βFtz-f1抑制边缘细胞中的JNK信号传导，从而下调管腔形成期间的细胞粘附。
During development, cells undergo multiple, distinct morphogenetic processes to form a tissue or organ, but how their temporal order and time interval are determined remain poorly understood. Here we show that the nuclear receptors E75 and DHR3 regulate the temporal order and time interval between the collective migration and lumen formation of a coherent group of cells named border cells duringDrosophilaoogenesis. We show that E75, in response to ecdysone signaling, antagonizes the activity of DHR3 during border cell migration, and DHR3 is necessary and sufficient for the subsequent lumen formation that is critical for micropyle morphogenesis. DHR3's lumen-inducing function is mainly mediated through βFtz-f1, another nuclear receptor and transcription factor. Furthermore, both DHR3 and βFtz-f1 are required for chitin secretion into the lumen, whereas DHR3 is sufficient for chitin secretion. Lastly, DHR3 and βFtz-f1 suppress JNK signaling in the border cells to downregulate cell adhesion during lumen formation.