Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma.
Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma.
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DOI：
10.3748/wjg.v17.i10.1373
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发表时间：
2011-03
影响因子：
4.3
通讯作者：
Jing-hua Zhou;Bin Zhang;K. Kernstine;L. Zhong
中科院分区：
文献类型：
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作者：
Jing-hua Zhou;Bin Zhang;K. Kernstine;L. Zhong
目的探讨血清抗基质金属蛋白酶-7(MMP7)自身抗体对食管鳞癌的诊断价值。方法从食管鳞癌组织中克隆基质金属蛋白酶-7基因，并在原核系统中进行表达和纯化。然后用反向捕获酶联免疫吸附试验(ELISA)检测50例原发ESCC患者和58例风险匹配的对照组血清中的MMP-7自身抗体。此外，还检测了38例胃癌患者和对照血清中的基质金属蛋白酶-7自身抗体水平。结果重组MMP7蛋白表达的最佳条件为：0.04mmo1/L异丙基-β-D-硫代半乳糖苷，37℃诱导4h。鳞癌患者血清中抗MMP7抗体水平显著高于对照组(OD450=1.69±0.08vsOD450=1.55±0.10，P<；0.001)。受试者工作特征(ROC)曲线下面积为0.87。当OD450>1.65时，诊断食管鳞癌的敏感性为78.0%，特异性为81.0%。尽管胃癌患者血清中抗MMP7自身抗体水平也显著高于对照组(OD450=1.62±0.06vsOD450=1.55±0.10，P<；0.001)，但其诊断准确性不如食管鳞癌患者显著。以OD450为临界值1.60，ROC曲线面积为0.75，灵敏度为60.5%，特异度为71.7%。结论血清基质金属蛋白酶-7自身抗体水平可作为诊断食管鳞癌的良好指标。
AIM To evaluate the diagnostic values of serum autoantibodies against matrix metalloproteinase-7 (MMP-7) in patients with esophageal squamous cell carcinoma (ESCC). METHODS The MMP-7 cDNA was cloned from ESCC tissues, and MMP-7 was expressed and purified from a prokaryotic system. MMP-7 autoantibodies were then measured in sera from 50 patients with primary ESCC and 58 risk-matched controls, using a reverse capture enzyme-linked immunosorbent assay (ELISA) in which autoantibodies to MMP-7 bound to the purified MMP-7 proteins. In addition, MMP-7 autoantibody levels in sera from 38 gastric cancer patients and from control serum samples were also tested. RESULTS The optimum conditions for recombinant MMP-7 protein expression were determined as 0.04 mmol/L Isopropyl-β-D-Thiogalactopyranoside (IPTG) induction at 37°C for four hours. The levels of serum autoantibodies against MMP-7 were significantly higher in patients with ESCC than in the matched-control samples (OD450 = 1.69 ± 0.08 vs OD450 = 1.55 ± 0.10, P < 0.001). The area under the receiver operating characteristic (ROC) curve was 0.87. The sensitivity and specificity for detection of ESCC were 78.0% and 81.0%, respectively, when the OD450 value was greater than 1.65. Although the levels of autoantibodies against MMP-7 were also significantly higher in patients with gastric cancer compared to control samples (OD450 = 1.62 ± 0.06 vs OD450 = 1.55 ± 0.10, P < 0.001), the diagnostic accuracy was less significant than in ESCC patients. The area of ROC curve was 0.75, whereas the sensitivity and specificity were 60.5% and 71.7%, respectively, when the cut-off value of OD450 was set at 1.60. CONCLUSION Serum autoantibody levels of MMP-7 may be a good diagnostic biomarker for esophageal squamous cell carcinoma.