In vivo function and evolution of the eutherian-specific pluripotency marker UTF1.
In vivo function and evolution of the eutherian-specific pluripotency marker UTF1.
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DOI：
10.1371/journal.pone.0068119
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Okuda A
中科院分区：
文献类型：
--
作者：
Nishimoto M;Katano M;Yamagishi T;Hishida T;Kamon M;Suzuki A;Hirasaki M;Nabeshima Y;Nabeshima Y;Katsura Y;Satta Y;Deakin JE;Graves JA;Kuroki Y;Ono R;Ishino F;Ema M;Takahashi S;Kato H;Okuda A
胎盘哺乳动物的胚胎发生是由胚胎本身和胎盘之间的微妙相互作用维持的。UTF1是在两种细胞谱系中表达的发育调节基因。在这里，我们分析了UTF1基因在小鼠发育过程中丢失的后果。我们发现，纯合子UTF1突变的新生小鼠显着小于野生型或杂合子突变小鼠，这表明，胎盘功能不全所造成的UTF1表达在胚胎外外胚层细胞的损失，至少部分促成了这种表型。我们还发现，胚胎干细胞中UTF1表达缺失对其多能性的影响非常微妙。基因组结构和序列比较显示UTF1基因仅存在于胎盘哺乳动物中。我们对UTF1同源基因家族的分析揭示了胎盘哺乳动物进化UTF1基因的可能机制。
Embryogenesis in placental mammals is sustained by exquisite interplay between the embryo proper and placenta. UTF1 is a developmentally regulated gene expressed in both cell lineages. Here, we analyzed the consequence of loss of the UTF1 gene during mouse development. We found that homozygous UTF1 mutant newborn mice were significantly smaller than wild-type or heterozygous mutant mice, suggesting that placental insufficiency caused by the loss of UTF1 expression in extra-embryonic ectodermal cells at least in part contributed to this phenotype. We also found that the effects of loss of UTF1 expression in embryonic stem cells on their pluripotency were very subtle. Genome structure and sequence comparisons revealed that the UTF1 gene exists only in placental mammals. Our analyses of a family of genes with homology to UTF1 revealed a possible mechanism by which placental mammals have evolved the UTF1 genes.
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影响因子：
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