Protective effect of Aframomum melegueta phenolics against CCl₄-induced rat hepatocytes damage; role of apoptosis and pro-inflammatory cytokines inhibition.
Protective effect of Aframomum melegueta phenolics against CCl₄-induced rat hepatocytes damage; role of apoptosis and pro-inflammatory cytokines inhibition.
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DOI：
10.1038/srep05880
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发表时间：
2014-07-30
影响因子：
4.6
通讯作者：
Hattori M
中科院分区：
文献类型：
--
作者：
El-Halawany AM;El Dine RS;El Sayed NS;Hattori M
黑胡椒是一种常用的非洲香料。通过保肝活性检测，从白木香种子氯仿提取物中分离得到一个新的二芳基庚烷类化合物，命名为3-（S）-乙酰基-1-（4′-羟基-3 ′，5′-二甲氧基苯基）-7-（3″，4 ″，5″-三羟基苯基）庚烷（1），以及两个新的羟基苯基烷酮，[8]-脱氢姜二酮（2）和[6]-脱氢paradol（3）。A.在大鼠肝细胞模型中使用四氯化碳（CCl 4）诱导的肝损伤研究了黄芪甲醇提取物、亚组分和分离的化合物。用甲醇、氯仿提取物和化合物1、5、8、9对A.黄芪能显著抑制CCl 4中毒引起的血清丙氨酸氨基转移酶（ALT）、硫代巴比妥酸反应物质（TBARS）、肿瘤坏死因子（TNFα）、白细胞介素1 β（IL-1β）、半胱天冬酶3和9的升高，并能显著提高肝脏谷胱甘肽（GSH）的还原水平。这些结果表明，黄芪提取物和分离的化合物对CCl 4诱导的急性肝损伤具有保护作用，这可能是由于提高抗氧化防御能力，抑制炎症反应和肝组织凋亡。
Aframomum melegueta is a commonly used African spice. Through a hepatoprotective bioassay-guided isolation, the chloroform fraction of A.melegueta seeds yielded one new diarylheptanoid named 3-(S)-acetyl-1-(4′-hydroxy-3′, 5′-di methoxyphenyl)-7-(3″,4″, 5″-trihydroxyphenyl)heptane (1), and two new hydroxyphenylalkanones, [8]-dehydrogingerdione (2) and [6]-dehydroparadol (3), in addition to six known compounds (4–9). The hepatoprotective effect of A. melegueta methanol extract, sub-fractions and isolated compounds was investigated using carbon tetrachloride (CCl4)-induced liver injury in a rat hepatocytes model. The methanol, chloroform extracts and compounds 1, 5, 8 and 9 of A. melegueta significantly inhibited the elevated serum alanine aminotransferase (ALT), thiobarbituric acid reactive substances (TBARS), tumor necrosis factor (TNFα), interleukin-1beta (Il-1β), caspase3 and 9 and enhanced the reduced liver glutathione (GSH) level caused by CCl4 intoxication. These results indicate that A.melegueta extracts, and isolated compounds play a protective role in CCl4 induced acute liver injury which might be due to elevated antioxidative defense potentials, suppressed inflammatory responses and apoptosis of liver tissue.