Localization of arginine vasopressin-neurophysin II messenger ribonucleic acid in the hypothalamus of control and Brattleboro rats by hybridization histochemistry with a synthetic pentadecamer oligonucleotide probe.

Localization of arginine vasopressin-neurophysin II messenger ribonucleic acid in the hypothalamus of control and Brattleboro rats by hybridization histochemistry with a synthetic pentadecamer oligonucleotide probe.
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通过使用合成的十五聚体寡核苷酸探针进行杂交组织化学,对对照大鼠和布拉特尔伯勒大鼠下丘脑中的精氨酸加压素-神经生理素 II 信使核糖核酸进行定位。

DOI:
10.1210/endo-116-6-2366
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发表时间:
1985
期刊:
影响因子:
4.8
通讯作者:
J. Funder
J. Funder
中科院分区:
医学2区
文献类型:
--
作者:
P. Fuller;J. Clements;J. Funder

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通过32p标记的寡核苷酸探针原位DNA-RNA杂交,我们确定了大鼠下丘脑中精氨酸抗利尿素-神经physin II (AVP-NPII)信使RNA (MRNA)的分布。在Sprague-Dawley大鼠和Brattleboro大鼠的视上核、视交叉上核和室旁核中发现了特异性强的杂交。这一AVP-NPII mRNA在Brattleboro和对照下丘脑中的等效分布的发现,证实并扩展了最近通过Northern blot分析报道的等效水平的AVP-NPII mRNA。
By using 32P-labeled oligonucleotide probes for in situ DNA-RNA hybridization, we have defined the distribution of messenger RNA (MRNA) for arginine vasopressin-neurophysin II (AVP-NPII) in the rat hypothalamus. With a specific pentadecamer nucleotide probe, strong specific hybridization is seen in supraoptic, suprachiasmatic, and paraventricular nuclei in both Sprague-Dawley and Brattleboro rats. This finding, of equivalent distribution of AVP-NPII mRNA in Brattleboro and control hypothalami, confirms and extends the recent report of equivalent levels of AVP-NPII mRNA by Northern blot analysis.
突变基因控制区域的化学合成和生物学研究。
DOI: 10.1101/sqb.1983.047.01.048
发表时间: 1983
期刊: Cold Spring Harbor symposia on quantitative biology
影响因子: --
作者:
Caruthers,MH;Beaucage,SL;Efcavitch,JW;Fisher,EF;Goldman,RA;deHaseth,PL;Mandecki,W;Matteucci,MD;Rosendahl,MS;Stabinsky,Y
通讯作者: Stabinsky,Y
大鼠甲状腺中降钙素信使核糖核酸的表征和定位。
DOI: 10.1210/endo-113-5-1616
发表时间: 1983
期刊: Endocrinology
影响因子: 4.8
作者:
Jacobs,JW;Simpson,E;Penschow,J;Hudson,P;Coghlan,J;Niall,H
通讯作者: Niall,H