KINETOCHORES AND MICROTUBULES IN MULTIPOLAR MITOSIS AND CHROMOSOME ORIENTATION
KINETOCHORES AND MICROTUBULES IN MULTIPOLAR MITOSIS AND CHROMOSOME ORIENTATION
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DOI：
10.1016/0014-4827(75)90140-8
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发表时间：
1975-01-01
影响因子：
3.7
通讯作者：
HENEEN, WK
中科院分区：
文献类型：
--
作者：
HENEEN, WK
本文研究了大鼠袋鼠细胞(PT-K1)多极分裂过程中动粒的超微结构和动粒微管的定位。中期着丝粒呈片状结构，大部分染色体呈两极微管取向。在某些染色体上观察到多极动粒取向。染色单体的动粒和相应数量的动粒微管的相等或不相等部分可以定向到两个不同的极点。在染色体附近观察到弯曲或弯曲的连续微管，呈多极取向。这一发现符合奥斯特格伦的“自动定向”理论[8]。推测在双极有丝分裂的完全中期之前，一个染色单体着丝粒向多个极的定向可能是染色体定向过程中的一种规律。
The ultrastructure of the kinetochore and the orientation of kinetochore microtubules were studied in multipolar divisions of cultured rat-kangaroo cells (Pt-K1). The metaphase kinetochore exhibited a lamellar structure and most of the chromosomes expressed a bipolar microtubule orientation. A multipolar kinetochore orientation was observed in some chromosomes. Equal or unequal portions of a chromatid's kinetochore and a corresponding number of kinetochore microtubules may be oriented to two different poles. Curved or bent continuous microtubules were observed in the vicinity of chromosomes showing multipolar orientation. The findings are in accordance with Östergren's theory of ‘auto-orientation’ [8]. It is speculated that orientation of a chromatid kinetochore to more than one pole might be a possible regular event during the process of chromosome orientation prior to full metaphase in bipolar mitosis.