Organization of the KpnI family of chromosomal distal-end satellite DNAs in Silene latifolia
Organization of the KpnI family of chromosomal distal-end satellite DNAs in Silene latifolia
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DOI：
10.1007/s10265-003-0106-6
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发表时间：
2003-08-01
影响因子：
2.8
通讯作者：
Kawano, S
中科院分区：
文献类型：
--
作者：
Kazama, Y;Sugiyama, R;Kawano, S
宽叶蝇子草（Silene latifolia）的主要卫星DNA由位于染色体末端的重复序列X43.1、RMY 1和SacI家族成员代表。目的：研究S. Latifolia（S1-远端-satDNA）中，我们分离了一个细菌人工染色体克隆（编号151312），该克隆含有多个具有Kpn I限制性位点的重复序列，并将该序列的一部分亚克隆到质粒载体中。测序分析证实，在插入的DNA中，Kpn I的识别或简并位点以310-324 bp的间隔重复26次。用26个Kpn I重复单位构建的系统发育树包含独立于SacI家族的簇状分支。很明显，Kpn I重复序列属于与SacI家族不同的Sl-远端-satDNA家族。在SacI家族中没有位点的限制性内切酶之后，我们将该家族命名为“Kpn I”。多色荧光原位杂交与Kpn I家族和RMY 1探针在高严格条件下进行。结果表明，7号染色体是独特的，它只在一端携带Kpn I家族。
The major satellite DNAs of the dioecious plant Silene latifolia are represented by the repetitive sequences X43.1, RMY1 and members of the SacI family, which are located at the distal ends of chromosomes. To characterize the satellite DNAs at the distal ends of the chromosomes in S. latifolia (Sl-distal-satDNA), we isolated a bacterial artificial chromosome clone (number 151312) that contained multiple repeat sequences with KpnI restriction sites, and subcloned a portion of this sequence into a plasmid vector. Sequencing analysis confirmed that recognition or degenerate sites for KpnI were repeated 26 times at intervals of 310-324 bp in the inserted DNA. The phylogenetic tree that was constructed with the 26 KpnI repeat units contained clustered branches that were independent of the SacI family. It is clear that the KpnI repeat belongs to an Sl-distal-satDNA family that is distinct from the SacI family. We designated this family as "KpnI" after the restriction enzyme that does not have a site in the SacI family. Multicolored fluorescent in situ hybridization was performed with the KpnI family and RMY1 probes under high stringency conditions. The results suggest that chromosome 7 is unique and that it carries the KpnI family at only one end.