Alternative Splicing Modulates Stem Cell Differentiation
Alternative Splicing Modulates Stem Cell Differentiation
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DOI：
10.3727/096368909x471260
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Lin, Shinn-Zong
中科院分区：
文献类型：
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作者：
Fu, Ru-Huei;Liu, Shih-Ping;Lin, Shinn-Zong
干细胞具有惊人的潜能，可以发育成许多不同的细胞类型。因此，主要的研究工作集中在干细胞和/或衍生祖细胞的移植，以恢复退化性疾病中耗尽的病变细胞。了解干细胞谱系分化过程中出现的分子控制，包括选择性剪接，对于开发再生医学中的干细胞治疗方法至关重要。选择性剪接允许单个基因编码具有不同蛋白质编码序列和RNA调控元件的多个转录本，增加了基因组的复杂性。利用选择性剪接的差异作为分子标记可能比简单的基因表达在不同程度的干细胞分化中更敏感。此外，选择性剪接可能为获得诱导多能干细胞或促进细胞间转分化提供新的思路，为恢复性治疗和基础医学研究提供新的思路。在这篇综述中，我们重点介绍了干细胞及其祖细胞中选择性剪接调控的最新进展。它有望为实现干细胞生物学及其相关应用提供急需的知识。
Stem cells have the surprising potential to develop into many different cell types. Therefore, major research efforts have focused on transplantation of stem cells and/or derived progenitors for restoring depleted diseased cells in degenerative disorders. Understanding the molecular controls, including alternative splicing, that arise during lineage differentiation of stem cells is crucial for developing stem cell therapeutic approaches in regeneration medicine. Alternative splicing to allow a single gene to encode multiple transcripts with different protein coding sequences and RNA regulatory elements increases genomic complexities. Utilizing differences in alternative splicing as a molecular marker may be more sensitive than simply gene expression in various degrees of stem cell differentiation. Moreover, alternative splicing maybe provide a new concept to acquire induced pluripotent stem cells or promote cell-cell transdifferentiation for restorative therapies and basic medicine researches. In this review, we highlight the recent advances of alternative splicing regulation in stem cells and their progenitors. It will hopefully provide much needed knowledge into realizing stem cell biology and related applications.