Differential transmission of actin motion within focal adhesions
Differential transmission of actin motion within focal adhesions
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DOI：
10.1126/science.1135085
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发表时间：
2007-01-05
期刊：
影响因子：
56.9
通讯作者：
Waterman-Storer, Clare M.
中科院分区：
文献类型：
--
作者：
Hu, Ke;Ji, Lin;Waterman-Storer, Clare M.
细胞迁移需要肌动蛋白细胞骨架中产生的运动通过粘着斑中蛋白质的复杂组装传递到细胞外环境。我们开发了相关的荧光斑点显微镜来测量耦合的焦点粘附蛋白肌动蛋白丝。不同类别的局灶性粘附结构和调节分子表现出不同程度的相关运动与肌动蛋白丝，表明层次传输肌动蛋白运动通过局灶性粘附。黏着斑蛋白，塔林蛋白和肌动蛋白丝之间的相互作用似乎构成了细胞骨架和整合素之间的滑动界面，产生一个分子离合器，在细胞迁移的形态动力学转变过程中受到调节。
Cell migration requires the transmission of motion generated in the actin cytoskeleton to the extracellular environment through a complex assembly of proteins in focal adhesions. We developed correlational fluorescent speckle microscopy to measure the coupling of focal-adhesion proteins to actin filaments. Different classes of focal-adhesion structural and regulatory molecules exhibited varying degrees of correlated motions with actin filaments, indicating hierarchical transmission of actin motion through focal adhesions. Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, generating a molecular clutch that is regulated during the morphodynamic transitions of cell migration.