Functional and morphologic study of retinal hypoperfusion injury induced by bilateral common carotid artery occlusion in rats
Functional and morphologic study of retinal hypoperfusion injury induced by bilateral common carotid artery occlusion in rats
复制标题
双侧颈总动脉闭塞致大鼠视网膜低灌注损伤的功能及形态学研究
DOI：
10.1038/s41598-018-36400-5
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发表时间：
2019-01-14
影响因子：
4.6
通讯作者：
Jin, Ming
中科院分区：
文献类型：
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作者：
Qin, Yali;Ji, Meiqi;Jin, Ming
视网膜低灌注损伤是眼缺血综合征（ocular ischemic syndrome，OIS）的病理生理基础，常导致严重的视力丧失。本研究旨在通过双侧颈总动脉阻塞（BCCAO）建立大鼠视网膜慢性低灌注模型，观察其视网膜功能和形态学变化。结果发现，BCCAO后2周，模型大鼠视网膜动脉硬化，视网膜静脉轻度扩张，视网膜血管瘤、血管瘤、血管节段性充盈，无灌注区。模型大鼠荧光素眼底血管造影（FFA）显示视网膜循环时间明显延长，视网膜电图（ERG）显示各时间点a、B波潜伏期明显延长，振幅明显降低，眼灌注持续减少。视网膜神经节细胞（RGCs）凋亡明显，视网膜各层厚度明显变薄。以上结果提示BCCAO可引起大鼠视网膜低灌注损伤，为OIS的研究提供了理想的动物模型。
Retinal hypoperfusion injury is the pathophysiologic basis of ocular ischemic syndrome (OIS) which often leads to severe visual loss. In this study, we aimed to establish a rat model of retinal chronic hypoperfusion by bilateral common carotid artery occlusion (BCCAO) and observe changes in the retinal function and morphology. We found that model rats showed retinal arteriosclerosis, slight dilated retinal vein, small hemangiomas, hemorrhages, vascular segmental filling, and nonperfused areas after 2 weeks of BCCAO. In the model rats, the retinal circulation time was significantly prolonged by fluorescein fundus angiography (FFA), the latency of a and b waves was delayed and the amplitude was decreased significantly at each time point by electroretinogram (ERG), and the perfusion of the eyes continued to reduced. Morphologic and ultrastructural changes covered that the retinal ganglion cells (RGCs) presented obvious apoptosis and the thickness in the retinal layers were significantly thinner. Collectively, these findings suggested that BCCAO induced retinal hypoperfusion injury in the model rats, thus providing an ideal animal model for the study of OIS.