Truepera radiovictrix gen. nov., sp nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov.
Truepera radiovictrix gen. nov., sp nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov.
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DOI：
10.1016/j.femsle.2005.05.002
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发表时间：
2005-06-15
影响因子：
2.1
通讯作者：
da Costa, MS
中科院分区：
文献类型：
--
作者：
Albuquerque, L;Simoes, C;da Costa, MS
从亚速尔群岛圣米格尔岛的温泉径流中发现了两种分离株，属于“奇球菌/栖热菌”门的新属的新种。菌株 RQ-24(T) 和 TU-8 是该门内独特系统发育谱系的第一个培养代表。这些菌株形成橙色/红色菌落、球形细胞，具有约50℃的最佳生长温度、约7.5至9.5之间的最佳生长pH，并且在pH低于6.5或高于pH 11.2时不生长。这些生物体在不添加 NaCl 的复杂培养基中生长，但在含有 1.0% NaCl 的培养基中具有最大生长速率，并且在含有高达 6.0% NaCl 的培养基中生长。这些生物体具有极强的抗电离辐射能力； 60% 的细胞在 5.0 kGy 下存活。这些菌株具有化学有机营养性和需氧性；在有或没有硝酸盐作为电子受体和葡萄糖作为碳源和能量源的厌氧条件下，在栖热菌培养基中不生长，但将葡萄糖发酵成D-乳酸而不形成气体。这些生物体吸收多种糖、有机酸和氨基酸。脂肪酸主要是异支链和反异支链的；还发现长链 1,2 二醇的相对比例较低；甲基萘醌 8 (MK-8) 是主要的呼吸醌。未检测到肽聚糖。基于16S rRNA基因序列分析、生理、生化和化学分析，我们描述了一个新属的新种，以菌株RQ-24(T) (CIp 108686(T) = LMG 22925(T) = DSM 17093(T))为代表，我们建议将其命名为Truepera radiovictrix。我们还提出了 Trueperaceae 家族。十一月以适应这个新属。 (c) 2005 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Two isolates, belonging to a new species of a novel genus of the Phylum "Deinococcus/Thermus", were recovered from hot spring runoffs on the Island of Sao Miguel in the Azores. Strains RQ-24(T) and TU-8 are the first cultured representatives of a distinct phylogenetic lineage within this phylum. These strains form orange/red colonies, spherical-shaped cells, have an optimum growth temperature of about 50 degrees C, an optimum pH for growth between about 7.5 and 9.5, and do not grow at pH below 6.5 or above pH 11.2. These organisms grow in complex media without added NaCl, but have a maximum growth rate in media with 1.0 % NaCl and grow in media containing up to 6.0 % NaCl. The organisms are extremely ionizing radiation resistant; 60 % of the cells survive 5.0 kGy. These strains are chemoorganotrophic and aerobic; do not grow in Thermus medium under anaerobic conditions with or without nitrate as electron acceptor and glucose as a source of carbon and energy, but ferment glucose to D-lactate without formation of gas. The organisms assimilate a large variety of sugars, organic acids and amino acids. Fatty acids are predominantly iso- and anteiso-branched; long chain 1,2 diols were also found in low relative proportions; menaquinone 8 (MK-8) is the primary respiratory quinone. Peptidoglycan was not detected. Based on 16S rRNA gene sequence analysis, physiological, biochemical and chemical analysis we describe a new species of one novel genus represented by strain RQ-24(T) (CIp 108686(T) = LMG 22925(T) = DSM 17093(T)) for which we propose the name Truepera radiovictrix. We also propose the family Trueperaceae fam. nov. to accommodate this new genus. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.