Organization and function of microfilaments during late epiboly in zebrafish embryos
Organization and function of microfilaments during late epiboly in zebrafish embryos
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DOI：
10.1002/dvdy.20144
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发表时间：
2004-10-01
影响因子：
2.5
通讯作者：
Webb, SE
中科院分区：
文献类型：
--
作者：
Cheng, JC;Miller, AL;Webb, SE
我们报告说，在斑马鱼epiboly，三个F-肌动蛋白为基础的结构后，胚盘迁移过去的胚胎赤道。它们由两个环状的F-肌动蛋白结构和一个点状的肌动蛋白带组成，前者形成于胚盘的深层细胞和包被层边缘，后者形成于卵黄合胞体外层。用细胞松弛素B或钙螯合剂二溴-BAPTA处理导致所有这三种基于肌动蛋白的结构的破坏，分别导致外延的减缓或立即停滞，随后是卵黄细胞闭塞的失败和胚胎通过植物极区域的最终裂解。因此，我们认为，这些结构的功能在闭塞的植物部分的卵黄细胞在后期阶段的外包。这些新的结构，它们的调制Ca 2+，以及其他先前描述的F-肌动蛋白为基础的结构，整个epiboly的功能可能的作用进行了讨论。(C)2004 Wiley-Liss，Inc.
We report that, during epiboly in zebrafish, three F-actin-based structures appear only after the blastoderm migrates past the embryonic equator. They are composed of two ring-like F-actin structures that form at the deep cell and enveloping layer margins of the blastoderm and a punctate actin band that develops in the external yolk syncytial layer. Treatment with cytochalasin B or the calcium chelator dibromo-BAPTA results in the disruption of all three of these actin-based structures, leading to the slowing or immediate arrest of epiboly, respectively, followed by a failure of yolk cell occlusion and the eventual lysis of the embryo through the vegetal pole region. We suggest, therefore, that these structures function in the occlusion of the vegetal portion of the yolk cell during the latter stages of epiboly. Possible roles for these new structures, their modulation by Ca2+, as well as the functions of other previously described F-actin-based structures observed throughout epiboly, are discussed. (C) 2004 Wiley-Liss, Inc.