Staging of Sporadic Parkinson Disease-Related α-Synuclein Pathology: Inter- and Intra-Rater Reliability
Staging of Sporadic Parkinson Disease-Related α-Synuclein Pathology: Inter- and Intra-Rater Reliability
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散发性帕金森病相关 α-突触核蛋白病理学的分期：评估者间和评估者内的可靠性
DOI：
10.1097/01.jnen.0000171652.40083.15
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发表时间：
2005
影响因子：
3.2
通讯作者：
H. Braak
中科院分区：
文献类型：
--
作者：
C. Müller;R. D. de Vos;C. Maurage;D. Thal;M. Tolnay;H. Braak
散发性帕金森病（sPD）以α-突触核蛋白（α-syn）包涵体为特征。这种夹杂物的分布似乎与疾病进展和严重程度有关。我们提出并测试了一个简单的分期方案，该方案基于5个石蜡切片中α-syn免疫反应性的存在，这些石蜡切片加在一起包含多达8个易受伤害的脑区。根据评估切片中是否存在夹杂物，定义了sPD的α-syn病理学的六个阶段。来自5个不同机构的6名观察员仅根据书面指示对21个案件进行了评级。评分员的一致性非常显著，平均误差低于一个阶段。通过所有评分者之间的配对比较分析，评分者间和评分者内的可靠性也基本接近完美。我们认为，α-syn病理分期程序适用于常规神经病理学和脑库学。明确定义的α-突触病理学阶段可能有助于研究之间的可比性，也有助于区分sPD与其他突触核蛋白病。
Sporadic Parkinson disease (sPD) is characterized by α-synuclein (α-syn) inclusions. The distribution of such inclusions appears to relate to disease progression and severity. We propose and test a simple staging protocol based on the presence of α-syn immunoreactivity in 5 paraffin sections that, taken together, contain up to 8 vulnerable brain regions. Six stages of α-syn pathology reminiscent for sPD are defined based on the presence or absence of inclusions in the assessed sections. Six observers from 5 different institutions rated 21 cases on the basis of written instructions only. The agreement of the raters was highly significant with a mean error below one stage. Both inter- and intra-rater reliability were also substantial to almost perfect as analyzed by paired comparison between all raters. We propose that the staging procedure for α-syn pathology is suitable for application in routine neuropathology and brain banking. Clearly defined stages of α-synpathology might aid the comparability between studies and also help to distinguish sPD from other synucleinopathies.
影响因子：
6
作者：
Lippa, CF;Fujiwara, H;Trojanowski, JQ
通讯作者：
Trojanowski, JQ