YEAST PEPTIDE PHEROMONES, A-FACTOR AND ALPHA-FACTOR, ACTIVATE A COMMON RESPONSE MECHANISM IN THEIR TARGET-CELLS
YEAST PEPTIDE PHEROMONES, A-FACTOR AND ALPHA-FACTOR, ACTIVATE A COMMON RESPONSE MECHANISM IN THEIR TARGET-CELLS
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DOI：
10.1016/0092-8674(86)90808-1
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发表时间：
1986-12-26
期刊：
影响因子：
64.5
通讯作者：
SPRAGUE, GF
中科院分区：
文献类型：
--
作者：
BENDER, A;SPRAGUE, GF
我们表明，在酵母中的细胞类型特异性的信息素反应是完全由一个细胞合成的受体的种类。这两种受体-信息素相互作用在功能上是可互换的，并且涉及共同的细胞内信号的产生。特别地，我们发现提供α-因子受体或α-因子受体，其通常不表达任一受体或任何其它α-或α-特定的产品，允许响应适当的信息素。此外，在缺乏α-因子受体的细胞中提供α-因子受体是有利的。因子受体恢复这些细胞的交配能力。最后，信息素反应的一个方面通常是α因子作用所特有的。细胞-增加的来自α-特异性STE 3基因-也可以在α-假A细胞（Mat α 2 ste 3 ste 13）的因子处理，应答α-因子，并且还具有活性STE 3启动子。
We show that in yeast the cell type specificity of pheromone response is determined solely by the species of receptor that a cell synthesizes. The two receptor-pheromone interactions are functionally interchangeable and involve the creation of a common intracellular signal. In particular, we find that provision of a-factor receptor or .alpha.-factor receptor in mat.alpha.1 mutants, which normally do not express either receptor or any other a- or .alpha.-specific products, allows response to the appropriate pheromone. Moreover, provision of a-factor receptor in a cells lacking .alpha.-factor receptor restores mating competence to those cells. Finally, an aspect of pheromone response that is normally unique to a-factor action on .alpha. cells-increased transcription from the .alpha.-specific STE3 gene-can also be observed following .alpha.-factor treatment of pseudo-a cells (mat.alpha.2 ste3 ste13), special mutants that respond to .alpha.-factor and also have an active STE3 promoter.