Fabrication and characterization of Chinese giant salamander skin composite collagen sponge as a high-strength rapid hemostatic material
Fabrication and characterization of Chinese giant salamander skin composite collagen sponge as a high-strength rapid hemostatic material
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DOI：
10.1080/09205063.2018.1485815
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发表时间：
2019-03-04
影响因子：
3.6
通讯作者：
Li, Can
中科院分区：
文献类型：
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作者：
Luo, Jianlin;Meng, Yonglu;Li, Can
中国大鲵在我国古代具有很高的药用价值和悠久的临床应用历史。本研究从CGS皮肤中提取胶原蛋白，并采用冷冻干燥法制备胶原海绵。采用克里思氧化微纤化纤维素（TEMPO MFC）和京尼平改善胶原海绵的力学性能。对所得海绵的吸湿性、孔隙率、力学性能、止血性能、形貌和生物降解性进行了详细研究。结果表明，CGSS胶原为I型胶原，具有完整的三螺旋结构，所制备的海绵具有多孔结构和良好的止血性能，促凝率为53.28%。然而，CGSS胶原海绵显示出低的拉伸强度（TS）98.80KPa、压缩强度（CS）1.48KPa和断裂伸长率（E）4.72%。将2.5%的TEMPO-MFC加入到天然胶原海绵中，TS增加到284.80KPa，CS增加到3.95KPa，E增加到8.19%，TS增加到188.26%，CS增加到166.89%，E增加到73.52%。这主要是由于TEMPO MFC对胶原海绵的腔体和腔壁进行了物理填充，TEMPO MFC的羧基与胶原蛋白的氨基之间形成了稳定的化学键，有效地提高了海绵的增韧效果，并形成了良好的界面结合。随后的0.3%京尼平处理进一步提高TS至605.00KPa和CS至8.66KPa作为交联反应的结果。复合增强还提高了胶原海绵的抗降解能力和促凝率。结果表明，TEMPO-MFC增韧京尼平交联CGSS复合胶原海绵是一种具有良好应用前景的高强度快速止血材料。
Chinese giant salamander (CGS) has high medicinal value and long history of clinical use in ancient China. In this study, CGS skin (CGSS) collagen was extracted and purified to prepare collagen sponge by freeze-drying. TEMPO oxidized microfibrillated cellulose (TEMPO-MFC) and genipin were adopted to improve the mechanical properties of collagen sponge. The hygroscopicity, porosity, mechanical properties, hemostatic performance, morphology, and biodegradability of the resultant sponges were investigated in detail. The results indicated that CGSS collagen was type I collagen with intact triple-helical structure, and the prepared sponge had porous structure and excellent hemostatic performance with procoagulant ratio of 53.28%. However, the CGSS collagen sponge showed low tensile strength (TS) of 98.80KPa, compression strength (CS) of 1.48KPa, and elongation at break (E) of 4.72%. Incorporating 2.5% TEMPO-MFC into the native CGSS collagen sponge resulted in an increase of 188.26% in TS to 284.80KPa, 166.89% in CS to 3.95KPa, and 73.52% in E to 8.19%. The improvements were attributed to the physical filling of TEMPO-MFC in cavity and cavity wall of collagen sponge and the stable chemical linkage between carboxyl of TEMPO-MFC and amino group of collagen which effectively improved the toughening of sponge and formed good interface bonding, respectively. Subsequent 0.3% genipin treatment further improved the TS to 605.00KPa and the CS to 8.66KPa as a result of crosslinking reaction. Moreover, the composite reinforcing also improved the anti-degradation ability and procoagulant ratio of collagen sponge. All results suggested that the TEMPO-MFC toughened and genipin crosslinked CGSS composite collagen sponge is a promising rapid hemostatic material with high-strength and can be applicated in biomedical field.