Effective control of Leptosphaeria maculans increases importance of L. biglobosa as a cause of phoma stem canker epidemics on oilseed rape.
Effective control of Leptosphaeria maculans increases importance of L. biglobosa as a cause of phoma stem canker epidemics on oilseed rape.
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DOI：
10.1002/ps.7248
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发表时间：
2022-10
影响因子：
4.1
通讯作者：
Yong-Ju Huang;S. N. Sidique;Chinthani S. Karandeni Dewage;L. H. Gajula;G. Mitrousia;A. Qi;J. West;B. Fitt
中科院分区：
文献类型：
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作者：
Yong-Ju Huang;S. N. Sidique;Chinthani S. Karandeni Dewage;L. H. Gajula;G. Mitrousia;A. Qi;J. West;B. Fitt
研究背景油菜腐烂病是一种危害油菜的病害，由两个相关真菌种黑斑链霉菌(Leptosphaeria Maculans)和大叶链霉菌(L.biglobosa)引起。然而，以往的研究主要集中在斑点假单胞菌上，对大球藻的研究较少。这项工作提供了证据，证明了大球藻在英国遏制溃烂流行的重要性。结果利用种特异性定量聚合酶链式反应对斑点乳杆菌和大斑乳杆菌DNA进行了定量检测，结果表明，大斑乳杆菌在英国9个油菜品种上均可引起上部茎部病变和茎基部溃烂。上部茎的病损主要由大叶乳杆菌引起。对茎基溃烂病而言，感病品种Drakkar的大斑菌DNA含量高于大斑菌DNA，而携带抗病基因Rlm7的品种大斑菌DNA含量高于大斑菌DNA。在茎底部溃烂病中检出大球状乳杆菌的频率从2000年的14%上升到2013年的95%。大叶乳杆菌和黄斑乳杆菌的子囊孢子大多在同一天释放，从2010/2011年到2012/2013年生长季，空气样品中的大叶乳杆菌子囊孢子数量有所增加。结论有效控制黑斑病，增加了黑斑病的侵染率，造成严重的茎上部病变和茎基部溃烂，造成产量损失。大叶乳杆菌对茎腐病流行的重要性已不容忽视。要有效控制褐腐病的流行，需要同时针对斑点落叶菌和大斑点落叶藻。这篇文章受版权保护。版权所有。
BACKGROUND Phoma stem canker is a damaging disease of oilseed rape, caused by two related fungal species, Leptosphaeria maculans and L. biglobosa. However, previous work has mainly focussed on L. maculans and there has been little work on L. biglobosa. This work provides evidence of the importance of L. biglobosa to stem canker epidemics in the UK. RESULTS Quantification of L. maculans and L. biglobosa DNA using species-specific quantitative PCR showed that L. biglobosa caused both upper stem lesions and stem base cankers on nine oilseed rape cultivars in the UK. Upper stem lesions were mainly caused by L. biglobosa. For stem base cankers, there was more L. maculans DNA than L. biglobosa DNA in the susceptible cultivar Drakkar, while there was more L. biglobosa DNA than L. maculans DNA in cultivars with the resistance gene Rlm7 against L. maculans. Frequency of L. biglobosa detected in stem base cankers increased from 14% in 2000 to 95% in 2013. Ascospores of L. biglobosa and L. maculans were mostly released on the same days and the number of L. biglobosa ascospores in air samples increased from the 2010/2011 to 2012/2013 growing seasons. CONCLUSION Effective control of L. maculans increased infection by L. biglobosa, causing severe upper stem lesions and stem base cankers, leading to yield losses. The importance of L. biglobosa to phoma stem canker epidemics can no longer be ignored. Effective control of phoma stem canker epidemics needs to target both L. maculans and L. biglobosa. This article is protected by copyright. All rights reserved.