Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria patients living in the Central Region of Ghana
Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria patients living in the Central Region of Ghana
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DOI：
10.1186/s12936-017-1955-0
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发表时间：
2017-08-01
期刊：
影响因子：
3
通讯作者：
Amoah, Linda Eva
中科院分区：
文献类型：
--
作者：
Acquah, Festus K.;Obboh, Evans K.;Amoah, Linda Eva
背景资料：疟疾防治工作的最新进展导致越来越多的国家疟疾防治方案执行消灭前措施，并表明需要开发新的工具来跟踪和控制疟疾传播。了解传播的关键是监测流行率和对寄生虫性阶段的免疫反应，称为配子体，这是负责传播。性阶段特异性抗原Pfs 230和Pfs 48/45已被鉴定并显示为传播阻断抗体的靶点，但在缺乏融合伴侣的情况下，它们难以重组产生。已知分别含有传递阻断表位6C和C 0的Pfs 48/45和Pfs 230区域，在乳酸乳球菌表达系统中产生，并用于酶联免疫吸附测定，以确定95名疟疾患者的血清反应性生活在加纳中部地区。C 0在L.在不存在融合配偶体的情况下，使用简化的纯化方案纯化乳酸。血清阳性率L.乳酸菌产生的Pfs 48/45.6C和Pfs 230。在研究人群中的C 0分别为74.7和72.8%.Conclusions：一个显着的年龄依赖性增加抗体滴度观察到，这表明针对这些抗原的疫苗可以加强在自然感染的领域。
Background: Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner.Methods: Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana.Results: Pfs48/45.6C and Pfs230. C0 were successfully produced in L. lactis in the absence of a fusion partner using a simplified purification scheme. Seroprevalence for L. lactis-produced Pfs48/45.6C and Pfs230. C0 in the study population was 74.7 and 72.8%, respectively.Conclusions: A significant age-dependent increase in antibody titers was observed, which suggests a vaccine targeting these antigens could be boosted during a natural infection in the field.