Stripe-patterned Microtunnel Device for Evaluating Acceleration of Conduction Velocity in Neuron and Schwann Cell Myelinating Coculture
Stripe-patterned Microtunnel Device for Evaluating Acceleration of Conduction Velocity in Neuron and Schwann Cell Myelinating Coculture
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DOI：
10.18494/sam.2018.2051
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发表时间：
2018-01-01
影响因子：
1.2
通讯作者：
Jimbo, Yasuhiko
中科院分区：
文献类型：
--
作者：
Sakai, Koji;Shimba, Kenta;Jimbo, Yasuhiko
了解髓鞘成熟的功能贡献在周围神经疾病的治疗中是必不可少的。然而，鲜为人知的是，在髓鞘成熟过程中的动作电位传导速度（CV）的顺序变化。这种知识的缺乏主要是因为难以评估包括在束中的单个轴突中的CV。在这项研究中，我们开发了一种神经元/雪旺细胞（SC）共培养装置，通过结合微隧道电极技术与表面图案化方法来评估髓鞘调节的CV变化。用于引导轴突生长的基质胶的条纹图案在由微隧道覆盖的电极组上对齐。免疫细胞化学染色表明，超过95%的细胞贴附到条纹图案和P0阳性髓鞘形成的微隧道覆盖的区域。电刺激诱发的活动在轴突和活动分别记录，表明我们的设备提供了CV在个别轴突。此外，在髓鞘形成开始后出现CV比无髓鞘轴突高两倍（1.02 m/s）的活性，表明可以检测到部分有髓鞘轴突的活性。这些结果表明，我们的方法对于研究CV随髓鞘成熟的顺序变化是有效的。
Understanding the functional contributions of myelin maturation is essential in the treatment of peripheral nerve diseases. However, little is known regarding sequential changes in the action-potential conduction velocity (CV) during the myelin maturation. This lack of knowledge is primarily because of the difficulty in evaluating the CV in individual axons included in the bundle. In this study, we developed a neuron/Schwann cell (SC) coculture device for evaluating the myelin-regulated changes in the CV by combining a microtunnel-electrode technique with a surface patterning method. A stripe pattern of matrigel for guiding axon growth was aligned on an electrode set covered by the microtunnel. Immunocytochemical staining demonstrated that more than 95% of the cells attached onto the stripe pattern and P0-positive myelin sheaths were formed in the microtunnel-covered area. Electrical stimulation evoked activities in the axons and the activities were separately recorded, indicating that our device provides the CV in individual axons. Furthermore, activity with the CV two times higher (1.02 m/s) than that of the unmyelinated axons appeared after the start of myelin formation, suggesting that the activity of partially myelinated axons can be detected. These results indicate that our approach is effective for investigating sequential changes in the CV with myelin maturation.