RORγt, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells
RORγt, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells
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DOI：
10.1016/s1074-7613(00)80645-7
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发表时间：
1998-12-01
期刊：
影响因子：
32.4
通讯作者：
Bevan, MJ
中科院分区：
文献类型：
--
作者：
He, YW;Deftos, ML;Bevan, MJ
我们已经鉴定了ROR γ t，一种新的胸腺特异性孤儿核受体ROR γ亚型，主要在CD 4（+）CD 8（+）双阳性胸腺细胞中表达。ROR γ t的异位表达通过抑制Pas配体的上调来保护T细胞杂交瘤免于活化诱导的细胞死亡。在杂交瘤刺激后，ROR γ t也抑制IL-2的产生，但不影响努尔-77和Egr-3的诱导，也不影响sing的上调。ROR γ t的配体结合和DNA结合结构域都是这种作用所必需的。我们提出，ROR γ t在未成熟胸腺细胞中表达的作用是抑制Pas配体表达和细胞因子分泌后，他们的TCR在积极或消极的选择。
We have identified ROR gamma t, a novel, thymus-specific isoform of the orphan nuclear receptor ROR gamma that is expressed predominantly in CD4(+) CD8(+) double-positive thymocytes. Ectopic expression of ROR gamma t protects T cell hybridomas from activation-induced cell death by inhibiting the upregulation of Pas ligand. Following hybridoma stimulation, ROR gamma t also inhibits IL-2 production but does not affect the induction of Nur-77 and Egr-3 nor the upregulation of sing. Both the ligand-binding and DNA-binding domains of ROR gamma t are required for this effect. We propose that the role of ROR gamma t expression in immature thymocytes is to inhibit Pas ligand expression and cytokine secretion following engagement of their TCR during positive or negative selection.