Immunoelectron Microscopic Study of Podoplanin Localization in Mouse Salivary Gland Myoepithelium
Immunoelectron Microscopic Study of Podoplanin Localization in Mouse Salivary Gland Myoepithelium
复制标题
DOI：
10.1267/ahc.10011
复制
发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Sawa, Yoshihiko
中科院分区：
文献类型：
--
作者：
Hata, Minoru;Amano, Ikuko;Sawa, Yoshihiko
我们最近报道，唾液腺细胞表达淋巴管内皮细胞标记podoplanin。本研究的目的是通过免疫组化方法研究肌上皮细胞标志物α-平滑肌肌动蛋白（SMA）在小鼠腮腺和舌下腺podoplanin阳性细胞上的表达，并通过免疫电镜研究阐明podoplanin在唾液腺肌上皮细胞中的定位。免疫荧光染色检测表达podoplanin和α-SMA的肌上皮细胞的分布，包埋前免疫电镜观察podoplanin抗体反应产物的定位。在免疫组化中，粘液腺泡末端部分和导管的表面被大量表达podoplanin的肌上皮细胞突起覆盖，并且抗podoplanin抗体对肌上皮细胞的免疫染色水平与抗α-SMA抗体的免疫染色水平完全一致。这些结果表明，podoplanin是一种唾液腺肌上皮细胞抗原，检测水平直接反映了肌上皮细胞的分布。在免疫电镜研究中，在舌下腺腺泡细胞之间的肌上皮细胞的胞质中发现了许多与抗podoplanin抗体结合的反应产物，并且在肌上皮细胞膜上也发现了这些反应产物。这些结果表明，唾液腺肌上皮细胞不断产生podoplanin和糖基化在高尔基体，并将它们运输到细胞膜。Podoplanin可能通过其作为粘蛋白型跨膜糖蛋白的特性参与维持肌上皮细胞的稳态。
We have recently reported that salivary gland cells express the lymphatic endothelial cell marker podoplanin. The present study was aimed to immunohistochemically investigate the expression of the myoepithelial cell marker alpha-smooth muscle actin (SMA) on podoplanin-positive cells in mouse parotid and sublingual glands, and to elucidate podoplanin localization in salivary gland myoepithelial cells by immunoelectron microscopic study. The distribution of myoepithelial cells expressing podoplanin and alpha-SMA was examined by immunofluorescent staining, and the localization of reaction products of anti-podoplanin antibody was investigated by pre-embedded immunoelectron microscopic method. In immunohistochemistry, the surfaces of both the mucous acini terminal portion and ducts were covered by a number of extensive myoepithelial cellular processes expressing podoplanin, and the immunostaining level with anti-podoplanin antibody to myoepithelial cells completely coincided with the immunostaining level with anti-alpha-SMA antibody. These findings suggest that podoplanin is a salivary gland myoepithelial cell antigen, and that the detection level directly reflects the myoepithelial cell distribution. In immunoelectron microscopic study, a number of reaction products with anti-podoplanin antibody were found at the Golgi apparatus binding to the endoplasmic reticulum in the cytoplasm of myoepithelial cells between sublingual gland acinar cells, and were also found at the myoepithelial cell membrane. These findings suggest that salivary gland myoepithelial cells constantly produce podoplanin and glycosylate at the Golgi apparatus, and transport them to the cell membrane. Podoplanin may be involved in maintaining the homeostasis of myoepithelial cells through its characteristic as a mucin-type transmembrane glycoprotein.