Depuration kinetics of murine norovirus in shellfish.
Depuration kinetics of murine norovirus in shellfish.
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贝类中鼠诺如病毒的净化动力学。
DOI：
10.1016/j.foodres.2014.06.027
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发表时间：
2014
影响因子：
8.1
通讯作者：
J. Romalde
中科院分区：
文献类型：
--
作者：
D. Polo;X. Feal;M. F. Varela;A. Monteagudo;J. Romalde
本研究评估并比较了小鼠诺如病毒（MNV-1）在马尼拉蛤（Venerupis philippinarum）和地中海贻贝（Mytilus galloprovincialis）中作为人类诺如病毒替代品的摄取率和净化动力学。试验10次，每次70 kg，每只软体动物5次。采用102pfu MNV-1/mL海水对软体动物进行24 h的生物积累控制。然后，将软体动物安置在实验净化系统中7天。利用TaqMan探针逆转录-实时PCR （RT-qPCR）对生物积累后的病毒污染进行定量，然后在净化过程中每天进行定量。在净化期结束时，进行感染性试验以检测感染性病毒颗粒的存在。结果表明，不同种类的软体动物对病毒的吸收和清除率存在显著差异。蛤和贻贝的平均病毒吸收量分别为5.4 × 106和4.0 × 105RNA拷贝/g，蛤的吸收量比贻贝高90%。与初始水平相比，蛤蜊和贻贝的平均降幅分别为60.5%和91.6%。另一方面，在两种双壳类动物中观察到MNV-1净化动力学的类似对数趋势线，有两个不同的阶段：在净化的前24-72小时内，病毒最初迅速减少，随后稳定下来，净化速度较慢。所有用蛤蜊和贻贝进行的试验都显示出病毒的显著减少，但在实验结束时，剩余的病毒仍具有传染性。
This study evaluates and compares the uptake rates and depuration kinetics of murine norovirus (MNV-1), as a human norovirus surrogate, in Manila clams (Venerupis philippinarum) and Mediterranean mussels (Mytilus galloprovincialis). Ten trials of 70 kg/trial (five with each mollusk) were performed. Mollusks were subjected to a controlled bioaccumulation step of 24 h with 102pfu MNV-1/mL seawater. Then, mollusks were relocated in an experimental depuration system for 7 days. Viral contamination was quantified after bioaccumulation and then daily during depuration by reverse transcription-real time PCR (RT-qPCR) with TaqMan probes. Infectivity assays were conducted to test the presence of infectious viral particles at the end of the depuration period. Results showed significant differences in the uptake and removal viral rates between molluscan species. The average viral uptake for clams and mussels were 5.4 × 106and 4.0 × 105RNA copies/g digestive tissue respectively, representing an uptake rate > 90% higher in clams. The average reductions with regard to the initial levels were 60.5% for clams and 91.6% for mussels. On the other hand, a similar logarithmic trend line in MNV-1 depuration kinetics was observed in both bivalves, with two differentiated phases: an initial rapid reduction of viruses during the first 24–72 h of depuration, and a subsequent stabilization with a slower depuration rate. All trials with clams and mussels showed significant viral reductions but remaining virus were still infectious at the end of the process.