Co-transfer of resistance to high concentrations of copper and first-line antibiotics among Enterococcus from different origins (humans, animals, the environment and foods) and clonal lineages
Co-transfer of resistance to high concentrations of copper and first-line antibiotics among Enterococcus from different origins (humans, animals, the environment and foods) and clonal lineages
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DOI：
10.1093/jac/dkt479
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发表时间：
2014-04-01
影响因子：
5.2
通讯作者：
Novais, Carla
中科院分区：
文献类型：
--
作者：
Silveira, Eduarda;Freitas, Ana R.;Novais, Carla
研究了来自革兰氏阳性菌的不同铜耐受基因的存在及其与抗生素耐药基因在不同来源肠球菌（Enterococcus，n922）中的共转移，包括来自人、动物、环境和食品样品的肠球菌（Enterococcus，n922）。通过标准程序进行抗菌药物和CuSO 4敏感性和接合试验，通过PCR进行Cu和抗生素耐药基因的细菌筛选，并通过PFGE/多位点序列分型进行克隆性。tcrB和cueO基因分别在15（n137/922）和14（n128/922）株分离株中出现，在猪中的出现率最高（P0.05）。它们在屎肠球菌中更常见（tcrB：23对8，在粪肠球菌中，12在其他物种中; cueO：25对5和9; P 0.05）。大多数E.屎肠菌（CuSO 4 MIC（50）24 mM，tcrB/cueO与CuSO 4 MIC（50）12 mM，tcrB/cueO），但不适用于其他物种。证明了铜耐受性（与tcrB、cueO或未知机制相关）与红霉素、四环素、万古霉素、氨基糖苷类或氨苄青霉素耐药性的共转移。在携带Cu耐受机制的分离株中检测到多种PFGE类型，其中一些在通常与人类感染相关的序列类型（ST）中鉴定（E。faecium和E.由于Cu化合物（例如防腐剂/动物饲料补充剂）的使用，Cu耐受性可能有助于选择/维持多重耐药性肠球菌（包括对用于治疗肠球菌感染的一线抗生素的抗性）。首次描述了多铜氧化酶cueO的分布以及氨苄青霉素抗性沿着耐铜基因的共转移。
We studied the occurrence of diverse copper (Cu) tolerance genes from Gram-positive bacteria and their co-transfer with antibiotic resistance genes among Enterococcus from diverse sources.Enterococcus (n922) of several species and from human, animal, environment and food samples were included. Antimicrobial and CuSO4 susceptibility and conjugation assays were performed by standard procedures, bacterial screening of Cu and antibiotic resistance genes by PCR, and clonality by PFGE/multilocus sequence typing.tcrB and cueO genes occurred in 15 (n137/922) and 14 (n128/922) of isolates, respectively, with the highest occurrence in piggeries (P0.05). They were more frequent among Enterococcus faecium (tcrB: 23 versus 8 in Enterococcus faecalis and 12 in other species; cueO: 25 versus 5 and 9, respectively; P0.05). A correlation between phenotypic and genotypic assays was observed for most E. faecium (CuSO4 MIC(50)24 mM in tcrB/cueO versus CuSO4 MIC(50)12 mM in tcrB/cueO), but not for other species. Co-transfer of Cu tolerance (associated with tcrB, cueO or an unknown mechanism) with erythromycin, tetracycline, vancomycin, aminoglycosides or ampicillin resistance was demonstrated. A variety of PFGE types was detected among isolates carrying Cu tolerance mechanisms, some identified in sequence types (STs) often linked to human infections (E. faecium from ST18 and ST78 clonal lineages and E. faecalis clonal complex 2).Cu tolerance might contribute to the selection/maintenance of multidrug-resistant Enterococcus (including resistance to first-line antibiotics used to treat enterococcal infections) due to the use of Cu compounds (e.g. antiseptics/animal feed supplements). The distribution of the multicopper oxidase cueO and the co-transfer of ampicillin resistance along with Cu tolerance genes are described for the first time.