Simulation of fungal-mediated cell death by fumonisin B1 and selection of fumonisin B1-resistant (fbr) Arabidopsis mutants
Simulation of fungal-mediated cell death by fumonisin B1 and selection of fumonisin B1-resistant (fbr) Arabidopsis mutants
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DOI：
10.1105/tpc.12.10.1811
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发表时间：
2000-10-01
期刊：
影响因子：
11.6
通讯作者：
Ausubel, FM
中科院分区：
文献类型：
--
作者：
Stone, JM;Heard, JE;Ausubel, FM
伏马菌素B1（FB 1），一个程序性细胞死亡诱导毒素产生的坏死型真菌植物病原体串珠镰刀菌，被用来模拟病原体感染拟南芥。植物渗入10 μ M FB 1和幼苗转移到琼脂培养基含有1 μ M FB 1发展病变的过敏反应，包括产生活性氧中间体，酚类化合物和胼胝质的沉积，积累的植物抗毒素，和表达的病程相关（PR）基因。通过将种子播种在含有1 μ M FB 1的琼脂上直接选择拟南芥FB 1抗性（fbr）突变体，在所述琼脂上野生型幼苗不能发育。选择用于进一步分析的两个突变体fbr 1和fbr 2响应于FB 1改变了PR基因表达。fbr 1和fbr 2对表达无毒基因avrRpt 2的无毒细菌病原体假单胞菌斑点致病变种（ES 4326）没有表现出差异抗性，但对缺乏无毒基因的毒性等基因菌株表现出增强的抗性。我们的研究结果表明，FB 1的实用程序的拟南芥防御相关的突变体的高通量分离，并建议病原体引起的程序性细胞死亡的宿主细胞可能是一个重要的功能兼容的植物-病原体相互作用。
Fumonisin B1 (FB1), a programmed cell death-eliciting toxin produced by the necrotrophic fungal plant pathogen Fusarium moniliforme, was used to simulate pathogen infection in Arabidopsis. Plants infiltrated with 10 muM FB1 and seedlings transferred to agar media containing 1 muM FB1 develop lesions reminiscent of the hypersensitive response, including generation of reactive oxygen intermediates, deposition of phenolic compounds and callose, accumulation of phytoalexin, and expression of pathogenesis-related (PR) genes. Arabidopsis FB1-resistant (fbr) mutants were selected directly by sowing seeds on agar containing 1 muM FB1, on which wild-type seedlings fail to develop. Two mutants chosen for further analyses, fbr1 and fbr2, had altered PR gene expression in response to FB1. fbr1 and fbr2 do not exhibit differential resistance to the avirulent bacterial pathogen Pseudomonas syringae pv maculicola (ES4326) expressing the avirulence gene avrRpt2 but do display enhanced resistance to a virulent isogenic strain that lacks the avirulence gene. Our results demonstrate the utility of FB1 for high-throughput isolation of Arabidopsis defense-related mutants and suggest that pathogen-elicited programmed cell death of host cells may be an important feature of compatible plant-pathogen interactions.