Genetic variation at the alpha-1-fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds
Genetic variation at the alpha-1-fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds
复制标题
亚洲野猪和中西方商品猪品种α-1-岩藻糖基转移酶（FUT1）基因的遗传变异
DOI：
10.1111/j.1439-0388.2008.00722.x
复制
发表时间：
2008-12-01
影响因子：
2.6
通讯作者：
Chen, G. H.
中科院分区：
文献类型：
--
作者：
Bao, W. B.;Wu, S. L.;Chen, G. H.
产肠毒素和/或志贺毒素的大肠杆菌F18（ETEC/STEC）是引起仔猪水肿病和断奶后腹泻的主要病原菌，α-1-岩藻糖基转移酶（FUT 1）基因已被确定为控制ETEC F18受体表达的候选基因。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对1个野猪品种、20个西方商品猪和中国地方猪种FUT 1基因开放阅读框307位核苷酸的遗传变异进行了研究。结果表明，FUT 1基因座仅在西方猪种和中国太湖猪（包括梅山猪、枫泾猪和二花脸猪）、淮猪和临高猪种中存在遗传多态性;仅杜洛克猪和皮特兰猪具有AA抗性基因型，而野猪和其他中国猪种仅具有GG易感基因型。结果表明，中国地方猪种缺乏对ETEC F18细菌产生抗性的遗传因素。ETEC F18抗性等位基因可能来源于欧洲野猪。由此推断，ETEC F18引起的仔猪水肿和断奶腹泻与生长速度密切相关，这可以解释为什么中国地方猪种对仔猪水肿和断奶腹泻的抗性强于西方猪种。
Escherichia coliF18 bacteria producing enterotoxins and/or shigatoxin (ETEC/STEC) are main pathogens that cause oedema disease and postweaning diarrhoea in piglets, and alpha‐1‐fucosyltransferase (FUT1) gene has been identified as a candidate gene for controlling the expression of ETEC F18 receptor. The genetic variations at nucleotide position 307 in open reading frame ofFUT1gene in one wild boar breed and 20 western commercial and Chinese native pig breeds were investigated by polymerase chain reaction–restriction fragment length polymorphism. The results showed that the genetic polymorphisms of theFUT1locus were only detected in western pig breeds and the Chinese Taihu (including Meishan pig, Fengjing pig and Erhualian pig), Huai and Lingao pig breeds; only Duroc and Pietrain possessed the resistant AA genotype, while the wild boar and other Chinese pig breeds only presented the susceptible genotype GG. The results indicated that Chinese native pig breeds lack genetic factors providing resistance to ETEC F18 bacteria. The resistant allele to ETEC F18 might originate from European wild boar. It was inferred that oedema and postweaning diarrhoea caused by ETEC F18 have close relationship with the growth rate, which can explain why on the contrary Chinese native pig breeds have stronger resistance to oedema and postweaning diarrhoea in piglets compared with western pig breeds.