IDENTIFICATION OF LYMPHOCYTES-T, MACROPHAGES, AND ACTIVATED EOSINOPHILS IN THE BRONCHIAL-MUCOSA IN INTRINSIC ASTHMA - RELATIONSHIP TO SYMPTOMS AND BRONCHIAL RESPONSIVENESS
IDENTIFICATION OF LYMPHOCYTES-T, MACROPHAGES, AND ACTIVATED EOSINOPHILS IN THE BRONCHIAL-MUCOSA IN INTRINSIC ASTHMA - RELATIONSHIP TO SYMPTOMS AND BRONCHIAL RESPONSIVENESS
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DOI：
10.1164/ajrccm/146.2.500
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发表时间：
1992-08-01
期刊：
影响因子：
--
通讯作者：
KAY, AB
中科院分区：
文献类型：
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作者：
BENTLEY, AM;MENZ, G;KAY, AB
应用免疫组织化学和一组单克隆抗体，我们比较了10例疾病严重程度相似的内源性(非过敏性)哮喘患者(IA)和7例外源性(过敏性)哮喘患者(EA)的支气管活检组织中T淋巴细胞、嗜酸性粒细胞、巨噬细胞和中性粒细胞的浸润情况。并与12例正常健康非特应性对照组(NC)进行比较。所有受试者都不吸烟，也没有口服或吸入皮质类固醇。与NC组比较，IA组CD45+细胞(总白细胞)、CD3+和CD4+淋巴细胞、CD68+巨噬细胞明显增多(分别为0.03、0.03、0.03和0.03)。IA和EA之间的CD4+(p<；0.05)和CD68+(p<；0.05)细胞数量也有增加。IA组IL-2受体阳性细胞(CD25+)、总嗜酸性粒细胞(MBP+)和活跃分泌嗜酸性粒细胞(EG2+)均高于NC组(P<0.01)。电针组EG2+嗜酸性粒细胞和CD25+(IL-2受体阳性)细胞也有类似的增加(分别为P<；0.01和P<；0.02)。三组弹性酶阳性细胞(中性粒细胞)的数量没有差异。IA组EG2+细胞数与AAS哮喘症状积分相关(r=0.65，p<；0.05)，而所有哮喘患者的EG2+细胞数(IA+EA)与气道乙酰甲胆碱反应性(r=-0.55，p<；0.03)和AAS哮喘症状积分(r=0.54，p<；0.03)相关。结果提示T淋巴细胞活化和嗜酸性粒细胞浸润是内源性和外源性哮喘的共同特征。
Using immunohistochemistry and a panel of monoclonal antibodies, we have compared T-lymphocyte, eosinophil, macrophage, and neutrophil infiltration in bronchial biopsies from 10 intrinsic (nonallergic) asthmatics (IA) and seven extrinsic (allergic) asthmatics (EA), with similar degrees of disease severity. The results were compared with 12 normal healthy nonatopic controls (NC). All subjects were nonsmokers and were not taking oral or inhaled corticosteroids. An intense mononuclear cell infiltrate was identified in IA with an increase in the number of CD45+ cells (total leukocytes), CD3+ and CD4+ lymphocytes, and CD68+ macrophages (p < 0.03, p < 0.01, p < 0.03, and p < 0.03, respectively), compared with NC. Increases were also found in CD4+ (p < 0.05) and CD68+ (p < 0.05) cell numbers between IA and EA. IL-2 receptor-bearing cells (CD25+) and the number of total (MBP+) and actively secreting (EG2+) eosinophils, were also increased in IA compared with NC (p < 0.01, p < 0.01, and p < 0.01, respectively). Similar increases in EG2+ eosinophils and CD25+ (IL-2 receptor-positive) cells were observed in EA (p < 0.01 and p < 0.02, respectively). No differences were detected in the three groups for the number of elastase-positive cells (neutrophils). EG2+ numbers in IA correlated with the Aas asthma symptom score (r = 0.65, p < 0.05), whereas EG2+ cell numbers in all asthmatics (IA + EA) correlated with airway methacholine responsiveness (r = -0.55, p < 0.03) and with the Aas asthma symptom score (r = 0.54, p < 0.03). The results suggest that T-lymphocyte activation and eosinophil infiltration are features common to both intrinsic and extrinsic asthma.