Photosynthetic Acclimation to Cold as a Potential Physiological Marker of Winter Barley Freezing Tolerance Assessed under Variable Winter Environment
Photosynthetic Acclimation to Cold as a Potential Physiological Marker of Winter Barley Freezing Tolerance Assessed under Variable Winter Environment
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DOI：
10.1111/j.1439-037x.2007.00292.x
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发表时间：
2008-02
影响因子：
3.5
通讯作者：
M. Rapacz;M. Tyrka;W. Kaczmarek;M. Gut;B. Wolanin;W. Mikulski
中科院分区：
文献类型：
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作者：
M. Rapacz;M. Tyrka;W. Kaczmarek;M. Gut;B. Wolanin;W. Mikulski
冬性是限制冬大麦栽培的一个复杂性状。vulgare）相对于温带气候的区域。在目前的研究中，我们验证了是否便宜和快速的生理标记表征光合适应冷可能提供强大的冬大麦基因型的特点，提高抗冻性。在田间试验和完全控制的条件下，对28个冬大麦品种和优良品系进行了3个冬季的抗寒性测定。为了提高耐冻性的环境变异性，部分植物在半控制条件下进行了去驯化，并在实验室进行了再驯化。研究了低温驯化后光系统II的表观量子效率（Fy/Fm）以及叶绿素荧光猝灭的光化学和非光化学系数（qp和NPQ）。在随后的几年里，耐冻性的现场实验室方法评估给出了不同的，甚至相反的结果。此外，去驯化与田间生长条件相互作用，每年对基因型进行不同的排名。结果表明，在实验室中测定的高水平的抗冻性，这是与光合适应冷可能是不够的表达的字段电阻，特别是当冬季条件不利于冷驯化。
Winter-hardiness is a complex trait limiting cultivation of winter barley (Hordeum vulgare ssp. vulgare) with respect to the regions of temperate climate. In the present studies, we verified whether inexpensive and fast physiological markers characterizing photosynthetic acclimation to cold may provide robust characteristics of winter barley genotypes for improved frost resistance. Freezing tolerance of 28 winter barley varieties and advanced breeding lines were tested for three winters in field-laboratory experiment and under fully controlled conditions. To increase the environmental variability of freezing tolerance, a part of the plants were also de-acclimated under semi-controlled conditions and re-acclimated in laboratory before freezing tests. After controlled cold acclimation, apparent quantum yield of photosystem II (F y /F m ) as well as photochemical (qp) and non-photochemical (NPQ) coefficients of chlorophyll fluorescence quenching were studied. Field-laboratory method assessment of freezing tolerance gives distinct and even opposite results in subsequent years. Also de-acclimation interacted with growth conditions in the field, giving different rankings of genotypes each year. The results obtained suggest that high level of freezing tolerance measured in laboratory, which is connected with photosynthetic acclimation to cold may be not sufficient for the expression of field resistance, especially when winter conditions are not favourable for cold acclimation.