MicroRNA machinery responds to peripheral nerve lesion in an injury-regulated pattern.
MicroRNA machinery responds to peripheral nerve lesion in an injury-regulated pattern.
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DOI：
10.1016/j.neuroscience.2011.06.017
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发表时间：
2011-09-08
期刊：
影响因子：
3.3
通讯作者：
Murashov, A. K.
中科院分区：
文献类型：
--
作者：
Wu, D.;Raafat, M.;Pak, E.;Hammond, S.;Murashov, A. K.
最近，已报道在用短干扰RNA (siRNA)转染的周围神经轴突中产生功能性和有效的RNA干扰(RNAi)。此外，在轴突型坐骨神经纤维以及体外再生的背根神经节 (DRG) 神经元中也鉴定出了 RNA 诱导的沉默复合物 (RISC) 的成分。基于这些观察结果，以及 siRNA 和 microRNA (miRNA) 共享相同效应酶的事实，我们假设内源 miRNA 生物合成途径会对周围神经损伤做出反应。为了回答这个问题，我们研究了小鼠周围神经挤压损伤后 miRNA 生物合成酶表达的变化。在这里，我们展示了几种关键的 miRNA 生物合成酶在体内坐骨神经和体外 DRG 轴突中以损伤调节模式表达。此外，坐骨神经损伤诱导mRNA加工体（P体）的表达，这是DRG轴突中mRNA降解的局部焦点。此外，通过 miRNA 微阵列鉴定了一组损伤调节的 miRNA，并通过 qPCR 和原位杂交分析进行了验证。综上所述，我们的数据支持了周围神经再生过程可能受 miRNA 通路调节的假设。
Recently, functional and potent RNA interference (RNAi) has been reported in peripheral nerve axons transfected with short-interfering RNA (siRNA). In addition, components of RNA-induced silencing complex (RISC) have been identified in axotomized sciatic nerve fibers as well as in regenerating dorsal root ganglia (DRG) neurons in vitro. Based on these observations, and on the fact that siRNA and microRNAs (miRNA) share the same effector enzymes, we hypothesized that the endogenous miRNA biosynthetic pathway would respond to peripheral nerve injury. To answer this question, we investigated changes in the expression of miRNA biosynthetic enzymes following peripheral nerve crush injury in mice. Here we show that several pivotal miRNA biosynthetic enzymes are expressed in an injury-regulated pattern in sciatic nerve in vivo, and in DRG axons in vitro. Moreover, the sciatic nerve lesion induced expression of mRNA-processing bodies (P-bodies), which are the local foci of mRNA degradation in DRG axons. In addition, a group of injury-regulated miRNAs was identified by miRNA microarray and validated by qPCR and in situ hybridization analyses. Taken together, our data support the hypothesis that the peripheral nerve regeneration processes may be regulated by miRNA pathway.
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影响因子：
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作者：
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影响因子：
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Didiot, Marie-Cecile;Subramanian, Murugan;Moine, Herve
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影响因子：
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