Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation
Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation
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DOI：
10.3177/jnsv.61.s78
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发表时间：
2015-05-01
影响因子：
1.6
通讯作者：
Miyazawa, Teruo
中科院分区：
文献类型：
--
作者：
Nakagawa, Kiyotaka;Kato, Shunji;Miyazawa, Teruo
越来越多的证据表明，过氧化磷脂酰胆碱（PCOOH）是氧化性食品变质和氧化性疾病的标志，这表明需要一个纯的PCOOH标准和可靠的定量方法。最近，我们合成了PCOOH异构体1-棕榈酰1-2-（9-氢过氧十八烯二烯基）- n-甘油-3-磷酸胆碱，（16:0/9-HpODE PC）和1-棕榈酰1-2-（13-氢过氧十八烯二烯基）- n-甘油-3-磷酸胆碱（16:0/13-HpODE PC）。结合液相色谱-串联质谱法，建立了一种可靠的定量方法。这篇小型综述描述了这些分析技术，特别强调临床样品分析。
Increasing evidence for phosphatidylcholine hydroperoxide (PCOOH) as a marker of oxidative food deterioration and oxidative diseases has revealed the need for a pure PCOOH standard and a reliable quantification method. Recently, we synthesized the PCOOH isomers 1-palmitoy1-2-(9-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine, (16:0/9-HpODE PC) and 1-palmitoy1-2-(13-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine (16:0/13-HpODE PC). Using these standards along with liquid chromatography-tandem mass spectrometry, a reliable quantification method was developed. This mini-review describes these analytical techniques, with a particular emphasis on clinical sample analysis.