Pyrimidine nucleoside salvage confers an advantage to Xenorhabdus nematophila in its host interactions.
Pyrimidine nucleoside salvage confers an advantage to Xenorhabdus nematophila in its host interactions.
复制标题
嘧啶核苷挽救赋予嗜线虫致病杆菌在宿主相互作用中的优势。
DOI：
10.1128/aem.71.10.6254-6259.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Goodrich-Blair,Heidi
中科院分区：
文献类型：
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作者：
Orchard,SamanthaS;Goodrich-Blair,Heidi
嗜食线虫是昆虫病原线虫的共生体，也是昆虫的病原菌。开始研究嘧啶补救在X.嗜水气单胞菌及其宿主，我们鉴定并突变了一个X。nematophila tdkhomologue.X.在平板试验中，嗜热菌tdk突变株对烟草天蛾的毒力降低，对线虫定殖的竞争性缺陷。野生型tdk等位基因的提供可转译突变株的缺陷。如大肠杆菌（Escherichia coli）、X.嗜热菌tdk编码脱氧胸苷激酶，其将回收的脱氧胸苷和脱氧尿苷核苷转化为它们各自的核苷酸形式。因此，核苷补救可能赋予X竞争优势。嗜虫性寄生在线虫的肠道中，对正常的昆虫致病性很重要。
Xenorhabdus nematophilais a mutualist of entomopathogenic nematodes and a pathogen of insects. To begin to examine the role of pyrimidine salvage in nutrient exchange betweenX. nematophilaand its hosts, we identified and mutated anX. nematophila tdkhomologue.X. nematophila tdkmutant strains had reduced virulence towardManduca sextainsects and a competitive defect for nematode colonization in plate-based assays. Provision of a wild-typetdkallele intranscorrected the defects of the mutant strain. As inEscherichia coli,X. nematophila tdkencodes a deoxythymidine kinase, which converts salvaged deoxythymidine and deoxyuridine nucleosides to their respective nucleotide forms. Thus, nucleoside salvage may confer a competitive advantage toX. nematophilain the nematode intestine and be important for normal entomopathogenicity.