CLONING OF A NOVEL BACTERIA-BINDING RECEPTOR STRUCTURALLY RELATED TO SCAVENGER RECEPTORS AND EXPRESSED IN A SUBSET OF MACROPHAGES
CLONING OF A NOVEL BACTERIA-BINDING RECEPTOR STRUCTURALLY RELATED TO SCAVENGER RECEPTORS AND EXPRESSED IN A SUBSET OF MACROPHAGES
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DOI：
10.1016/0092-8674(95)90514-6
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发表时间：
1995-02-24
期刊：
影响因子：
64.5
通讯作者：
TRYGGVASON, K
中科院分区：
文献类型：
--
作者：
ELOMAA, O;KANGAS, M;TRYGGVASON, K
在巨噬细胞亚群中发现了一种新的小鼠质膜蛋白。它有一个胞内N-末端结构域、一个跨膜结构域、一个带有短间隔区的胞外区、一个含有89个Gly-Xaa-Yaa重复序列的胶原区和一个C-末端富含半胱氨酸的结构域。原位杂交和免疫组织化学染色已将该蛋白定位于脾边缘带和淋巴结髓索的巨噬细胞亚群。肝、肺巨噬细胞均未见表达。转基因的COS细胞合成了一种天然的三聚体质膜蛋白，它能结合标记的细菌和乙酰化的低密度脂蛋白，但不能结合酵母或Ficoll。结果表明，该新蛋白是一种巨噬细胞特异性膜受体，在宿主防御中发挥作用，因为它在出生后在巨噬细胞中表达，巨噬细胞被认为与细菌抗原结合和吞噬作用密切相关。
A novel murine plasma membrane protein has been identified in subpopulations of macrophages. It has an intracellular N-terminal domain, a transmembrane domain, and an extracellular region with a short spacer, an 89 Gly-Xaa-Yaa repeat-containing collagenous domain, and a C-terminal cysteine-rich domain. In situ hybridization and immunohistochemical staining have localized the protein to a subset of macrophages in the marginal zone of the spleen and the medullary cord of lymph nodes. No expression was observed in macrophages of liver or lung. Transfected COS cells synthesized a native trimeric plasma membrane protein that bound labeled bacteria and acetylated LDL, but not yeast or Ficoll. The results suggest that the novel protein is a macrophage-specific membrane receptor with a role in host defense, as it shows postnatal expression in macrophages, which are considered responsible far the binding of bacterial antigens and phagocytosis.