Bacterial toxin-antitoxin gene system as containment control in yeast cells
Bacterial toxin-antitoxin gene system as containment control in yeast cells
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DOI：
10.1128/aem.66.12.5524-5526.2000
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发表时间：
2000-12-01
影响因子：
4.4
通讯作者：
Piskur, J
中科院分区：
文献类型：
--
作者：
Kristoffersen, P;Jensen, GB;Piskur, J
探索了细菌毒素-抗毒素基因系统在真核生物中用于遏制控制的潜力。在酿酒酵母中表达了大肠杆菌Rele和relB基因，该基因的表达对酵母细胞具有很强的毒性。然而，relB基因的表达在一定程度上抵消了Rele的作用，这表明在酿酒酵母中也存在毒素-抗毒素相互作用，因此细菌毒素-抗毒素基因系统在控制真核细胞的细胞增殖方面也有潜在的应用，特别是在工业发酵过程中，转基因细胞的逃逸被认为是高风险的。
The potential of a bacterial toxin-antitoxin gene system for use in containment control in eukaryotes was explored. The Escherichia coli relE and relB genes were expressed in the yeast Saccharomyces cerevisiae, Expression of the relE gene was highly toxic to yeast cells. However, expression of the relB gene counteracted the effect of relE to some extent, suggesting that toxin-antitoxin interaction also occurs in S. cerevisiae, Thus, bacterial toxin-antitoxin gene systems also have potential applications in the control of cell proliferation in eukaryotic cells, especially in those industrial fermentation processes in which the escape of genetically modified cells would be considered highly risky.