Allelic variation for a candidate gene for GS7, responsible for grain shape in rice
Allelic variation for a candidate gene for GS7, responsible for grain shape in rice
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负责水稻粒形的 GS7 候选基因的等位基因变异
DOI：
10.1007/s00122-012-1914-7
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发表时间：
2012-10-01
影响因子：
5.4
通讯作者：
Hu, Peisong
中科院分区：
文献类型：
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作者：
Shao, Gaoneng;Wei, Xiangjin;Hu, Peisong
粒形是决定稻米最终使用品质的重要因素。粒形QTL GS7的基因组定位被缩小到位于7号染色体4.8 kb片段内。cv中的同源区。Nipponbare未包含注释基因，但预测了两个开放阅读框，根据序列变异与表型的相关性，其中一个（ORF2）代表了GS7基因的可能候选基因。对ORF2转录进行半定量和定量RT-PCR分析，结果表明，该基因在玉米叶片和穗部均有活性。存在D50等位基因，但不存在cv。HB277等位基因。D50 GS7等位基因可能来源于热带粳稻基因库。对gs7和GS3交替等位基因粒长的影响表明，组合3/A型比组合1/A型籽粒长。在ORF2序列中开发的Indel标记可用于预测籽粒长度。
Grain shape is an important component of end-use quality in rice. The genomic location of the grain shape QTL GS7 was narrowed to lie within a 4.8-kb segment on chromosome 7. The homologous region in cv. Nipponbare contains no annotated genes, while two open reading frames were predicted, one of which (ORF2) represented a likely candidate for GS7 gene on the basis of correlation between sequence variation and phenotype. Semi-quantitative and quantitative RT-PCR analysis of ORF2 transcription showed that the gene was active in both the leaf and panicle when the cv. D50 allele was present, but not in the presence of the cv. HB277 allele. D50 GS7 allele may have originated from the tropical japonica genepool. The effect on grain length of the alternative alleles at GS7and GS3 showed that combination type 3/A was associated with longer grains than type 1/A. An Indel marker developed within the ORF2 sequence was informative for predicting grain length.